The complete chloroplast genome of Bretschneidera sinensis (Bretschneideraceae)
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TECHNICAL NOTE
The complete chloroplast genome of Bretschneidera sinensis (Bretschneideraceae) Songyi Huang1 · Qiujie Zhou2 · Haicen Shen1 · Zheng Chen1 · Qiaoming Zhu1 · Wei Wu2 Received: 13 October 2017 / Accepted: 15 November 2017 © Springer Science+Business Media B.V., part of Springer Nature 2017
Abstract Bretschneidera sinensis: Hemsl., a tree sparsely distributed in southern and southwestern China as well as northern Vietnam, is the only species of Bretschneideraceae. Being endangered in China, B. sinensis is one of the National Key Protected Wild Plants (Class I) and has been included in the China Plant Red Data Book. Here we reported its complete chloroplast genome sequence assembled from Illumina sequencing reads. The complete chloroplast genome was 159,004 bp long, consisting of a large single copy (LSC) region of 86,910 bp and a small single copy (SSC) region of 18,820 bp, separated by a pair of inverted repeat region (IRs) of 26,637 bp each. The chloroplast genome contained 132 genes, including 37 tRNA, 8 rRNA and 87 protein-coding genes. Phylogenetic analysis using the maximum likelihood method showed that B. sinensis is closest to Carica papaya among closely related species with available chloroplast genome sequences. Keywords Bretschneidera sinensis · Chloroplast genome · Illumina sequencing · Phylogenetic analysis Bretschneidera sinensis Hemsl., the only species of Bretschneideraceae, is a tree sparsely distributed in southern and southwestern China as well as northern Vietnam (Chen 1984). Being rare and endangered in China, B. sinensis was included in the China Plant Red Data Book (Fu 1992). It is also listed in one of the National Key Protected Wild Plants (Class I). To date, no studies have been conducted to comprehensively investigate its genetic variation across its range. The phylogenetic position of B. sinensis has been controversial for a long time. It was first described by Hemsley in 1901 and placed originally in the Sapindaceae family (Hemsley 1901). Pollen morphology analysis suggested that B. sinensis is closely related to Sapindaceae, Hippocastanaceae, Moringaceae, and Caesalpinioideae (Chaw and Peng 1987). Floral anatomy supported a close relationship with Akaniaceae and Tropaeolaceae (Ronse De Craene et al. 2002). On the basis of chloroplast rbcL sequences, a close affinity between Bretschneidera and the order Capparales * Wei Wu [email protected] 1
Guangzhou Landscaping Company, Guangzhou 510950, China
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
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was demonstrated and especially with the genus Tropaeolum (Rodman et al. 1993). Cladistic analyses based on the chloroplast rbcL sequences showed that the genus Akania clustered strongly with Bretschneidera and then Tropaeolum (Gadek et al. 1992). Further phylogenetic analysis based on the nuclear 18S rRNA gene and chloroplast rbcL gene yielded the same result (Rodman et al. 1998). All the Angios
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