The cultivation of Pyropia haitanensis has important impacts on the seawater microbial community
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23RD INTERNATIONAL SEAWEED SYMPOSIUM, JEJU
The cultivation of Pyropia haitanensis has important impacts on the seawater microbial community Wenlei Wang 1,2,3 & Lei Wu 1,2,3 & Kai Xu 1,2,3 & Yan Xu 1,2,3 & Dehua Ji 1,2,3 & Changsheng Chen 1,2,3 & Chaotian Xie 1,2,3 Received: 26 August 2019 / Revised and accepted: 5 February 2020 # The Author(s) 2020
Abstract Microorganisms play important roles in the growth and development of macroalgae. Still, the biodiversity of the epiphytic microbial community associated with the economically important red alga Pyropia haitanensis during the cultivation period remains uncharacterized, especially the effects of P. haitanensis cultivation on the microbial community of surrounding seawater. Here, we isolated epiphytic microbes from P. haitanensis during the thallus stage during oceanic cultivation and the conchocelis stage during industrial cultivation. The dynamic diversity patterns, as determined by 16S and 18S rRNA gene sequencing of the bacterial and fungal communities, respectively, associated with P. haitanensis and seawater in the presence and absence of algal cultivation were investigated. A notable distinction was observed between the microbial communities of seawater with and without P. haitanensis cultivation. Additionally, the alpha-diversity of seawater with P. haitanensis cultivation was significantly greater than without P. haitanensis cultivation. Cyanobacteria were the dominant species in the latter, while Rhodobacteraceae was enriched in the former. Furthermore, there were significant differences in the microbial community of P. haitanensis at the thallus and conchocelis stages. Seawater properties had significant direct effects on the microbial diversity of P. haitanensis and cultivation seawater, but not on non-cultivation seawater. The enriched microbial presence might promote thallus morphogenesis and be beneficial for the growth and development of both the thallus and conchocelis stages. These findings expand our knowledge of the bacteria and fungi that are beneficial for Pyropia nursery seeding and cultivation, as well as the effects of P. haitanensis cultivation on the seawater microbial community. Keywords Pyropia haitanensis . Microbial communities . Conchocelis stage . Thallus stage . Oceanic and industrial cultivation
Introduction Microbes are widely distributed in seawater and selectively enrich the phycosphere (Bell and Mitchell 1972). These microbes can form communities with unique functions and structures, which may play important roles in marine food webs and biogeochemical cycles (Cole 1982; Armstrong et al. 2001; Farooq and Francesca 2007). Additionally, host Wenlei Wang and Lei Wu contributed equally to this work. * Chaotian Xie [email protected] 1
Fisheries College, Jimei University, Xiamen 361021, China
2
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Xiamen 361021, China
3
Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen 361021, China
microbe interactions pl
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