The Cytochemical Bioassay of Polypeptide Hormones
The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays
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Volume 17
Edited by F. Gross, Heidelberg· M.M. Grumbach, San Francisco A. Labhart, Zurich . M.B. Lipsett, Bethesda T. Mann, Cambridge· L.T. Samuels (t), Salt Lake City J. Zander, Munchen
J. Chayen
The Cytochemical Bioassay of Polypeptide Hormones With 72 Figures and 7 Tables
Springer- Verlag Berlin Heidelberg New York 1980
Joseph Chayen Ph. D., D. Sc. Division of Cellular Biology The Mathilda and Terence Kennedy Institute of Rheumatology, London, UK
ISBN-13: 978-3-642-81461-7 e-ISBN-13: 978-3-642-81459-4 DOT: 10.1007/978-3-642-81459-4 Library of Congress Cataiq,ging in Publication Data. Chayen. Joseph. The cytochemical bioassay of polypeptide honnones. (Monographs on endocrinology; v. 17) Bibliography: p. Includes index. 1. Cytochemistry - Technique. 2. Biological assay. 3. Peptide
hormones. I. Title. [DNLM: 1. Biological assay. 2. Histocytochemistry. 3. Hormones - Analysis. 4. Peptides - Analysi5. WI M057 v. 17/ WK185 C512c] QH611.C45
574.19'2456
80-13680
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Springer-Verlag Berlin, Heidelberg 1980 Softcover reprint of the hardcover 1st edition 1980 The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Typesetting, printing and binding: Oscar Brandstetter Druckerei KG, Wiesbaden 2125/3020 - 543210
Preface
The cytochemical bioassay system was described in a short abstract in 1971, and more fully, in the cytochemical bioassay of corticotrophin, in 1972. Since then, cytochemical bioassays have been described for several polypeptide hormones, and these assays are already widely used. It is expedient that the subject should be reviewed, as it is in this monograph, by one writer who has had the good fortune to have taken part in the growth of cytochemistry from its early origins to its present position as the basis of possibly the most sensitive bioassay system currently available. However, it should be noted that major contributions have been made by many, both to the development of the subject and to the establishment of the bioassays. The object of this preface is to try to give some perspective to the growth of this subject and to record that the cytochemical bioassay system has been fostered by many outstanding scientists in an atmosphere of remarkable goodwill. To begin with, there could have been no cytochemical bioassays until cytochemistry had been converted from its rather unsure origins into a precise and quantitative form of cellular bioche
