The effects of calcium and glucose supplementation on bone of young female rats in case of disturbances in energy balanc
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POSTER PRESENTATION
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The effects of calcium and glucose supplementation on bone of young female rats in case of disturbances in energy balance caused by their food restriction and exercise Yuki Aikawa1*, Yuya Kakutani1, Ikuko Ezawa2, Naomi Omi1 From International Society of Sports Nutrition: 8th Annual ISSN Conference and Expo Las Vegas, NV, USA. 24-25 June 2011 Background Female athletes, with a strong awareness of their weight loss, are prone to restrict their food intake. A major concern arisen from such athletes’ daily training would be an imbalance of energy intake and energy expenditure which resulted in an osteoporotic fracture. Calcium (Ca) is a major mineral content in bone, otherwise Glucose (Glu) is an energy source. It is not clear whether Ca or Glu supplementation have a positive effect on bone in case of disturbances in energy balance caused by their food restriction and exercise. Methods 49 female Sprague-Dawley rats (age 8 weeks) were divided into 6 groups: ad libitum feeding (0.6% Ca diet) and non-exercise group [Cont group]; ad libitum feeding (0.6% Ca diet) and exercise group [Ex group]; food restriction (0.6% Ca diet)and exercise group [REx group]; food restriction, Ca supplementation (1.2% Ca diet) and exercise group [REx+Ca group]; food restriction (0.6% Ca diet), Glu supplementation and exercise group [REx+Glu group]; food restriction, Ca supplementation (1.2% Ca diet), Glu supplementation, exercise group [REx+Ca+Glu group]. They were reared in individual cages during 38 days. Food restriction was 70% of food intake of the Cont group. Exercise was voluntary wheel running. We measured the number of revolutions every day. After the treatment period, intra-abdominal fat, femur, lumbar spine and tibia were collected. * Correspondence: [email protected] 1 Laboratory of Sports Nutrition, University of Tsukuba Graduate School of Comprehensive Human Sciences, Tsukuba 305-8574, Japan Full list of author information is available at the end of the article
Statistical analysis was performed using ANOVA followed by a Scheffe’s post hoc comparisons test (p
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