The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation
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The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation Kei Hashimoto 1,2,3 & Kimiko Yamashita 1,2,4,5,6 & Kanako Enoyoshi 1,2 & Xavier Dahan 2,7 & Tatsu Takeuchi 8 & Hiroshi Kori 1,9 & Mari Gotoh 3 Received: 9 November 2019 / Accepted: 10 August 2020/ # The Author(s) 2020
Abstract
Motile human-skin fibroblasts form macroscopic swirling patterns when grown to confluence on a culture dish. In this paper, we investigate the effect of coating the culture-dish surface with collagen on the resulting pattern, using human-skin fibroblast NB1RGB cells as the model system. The presence of the collagen coating is expected to enhance the adherence of the fibroblasts to the dish surface, and thereby also enhance the traction that the fibroblasts have as they move. We find that, contrary to our initial expectation, the coating does not significantly affect the motility of the fibroblasts. Their eventual number density at confluence is also unaffected. However, the coherence length of cell orientation in the swirling pattern is diminished. We also find that the fibroblasts cultured in collagen-coated dishes are rounder in shape and shorter in perimeter, compared with those cultured in uncoated polystyrene or glass culture dishes. We hypothesise that the rounder cell-shape which weakens the cell–cell nematic contact interaction is responsible for the change in coherence length. A simple mathematical model of the migrating fibroblasts is constructed, which demonstrates that constant motility with weaker nematic interaction strength does indeed lead to the shortening of the coherence length. Keywords Pattern formation . Cell population . Fibroblast . Collagen . Mathematical model
1 Introduction Collective cell migration is a key process observed at various stages in the development of multicellular organisms, starting with gastrulation and continuing into organogenesis [1]. Well-studied examples include neural-tube closure of vertebrae and lateral-line formation in zebrafish. After birth, it is involved in wound healing and cancer metastasis [2]. Collective cell migration is also Kei Hashimoto, Kimiko Yamashita, and Kanako Enoyoshi contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10867-02009556-3) contains supplementary material, which is available to authorized users.
* Hiroshi Kori [email protected]–tokyo.ac.jp Extended author information available on the last page of the article
K. Hashimoto et al.
observed in single-cell organisms. A well-known example is aggregation by which Dictyostelium cells form a slug-like structure when starved [3]. Deciphering the mechanisms that drive robust and precise collective migration of a large number of cells is of vital importance in understanding development, differentiation, and evolution, with many possible applications in cancer therapies, regenerative medicine, and tissue engineering [2, 4–6]. In vitro cultivation studies can provide important insights into col
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