The Effects of Process-Induced Pectin Changes on the Viscosity of Carrot and Tomato Sera

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ORIGINAL PAPER

The Effects of Process-Induced Pectin Changes on the Viscosity of Carrot and Tomato Sera Katlijn R. N. Moelants & Ruben P. Jolie & Stijn K. J. Palmers & Ruth Cardinaels & Stefanie Christiaens & Sandy Van Buggenhout & Ann M. Van Loey & Paula Moldenaers & Marc E. Hendrickx

Received: 24 April 2012 / Accepted: 11 November 2012 # Springer Science+Business Media New York 2012

Abstract The influence of process-induced pectin changes on the kinematic viscosity of the serum phases of carrot and tomato purées was investigated in this study. Variations in the amount of pectin present in the serum phase and pectin’s fine structure were induced by subjecting carrot and tomato to different thermal treatments, i.e. a mild and a strong heat treatment, followed by blending and high-pressure homogenisation at different pressure levels (0, 20 and 100 MPa). Changes in pectin structure were monitored by determination of the degree of methoxylation, analysing the molar mass distribution and immuno-dot blotting using anti-pectin antibodies. Characterisation of serum pectins revealed that a strong thermal treatment caused pectin thermosolubilisation and depolymerisation, especially in carrot sera, and highpressure homogenisation provoked predominantly mechanical pectin solubilisation in carrot sera and pectin depolymerisation in tomato sera. It was observed that the kinematic viscosity of carrot sera was mainly affected by the amount of solubilised pectin, whereas in tomato sera, the kinematic viscosity could mainly be explained in terms of polysaccharide chain length. The degree of methoxylation of pectin, K. R. N. Moelants : R. P. Jolie : S. K. J. Palmers : S. Christiaens : S. Van Buggenhout : A. M. Van Loey : M. E. Hendrickx (*) Department of Microbial and Molecular Systems (M2S), Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 22, 2457, 3001 Leuven, Belgium e-mail: [email protected] R. Cardinaels : P. Moldenaers Department of Chemical Engineering, Laboratory of Applied Rheology and Polymer Processing, KU Leuven, Willem de Croylaan 46, 2423, 3001 Leuven, Belgium

which has a broader distribution in tomato sera than in carrot sera, seemed to have a less pronounced role in determining the kinematic viscosity. In general, it could be concluded that serum viscosity is mainly determined by the amount and size of the solubilised pectin. The influence of serum viscosity on the rheology of the studied purées was limited as the rheology of this type of system is largely determined by the particle properties of the dispersed phase. Keywords Serum . Pectin . Viscosity . Carrot . Tomato . High-pressure homogenization

Introduction Large amounts of fruits and vegetables are nowadays processed into liquid(-like) derived products such as soups, juices and purées. These systems consist of plant-tissuebased particles suspended in a continuous serum phase containing solubilised cell wall material, in particular solubilised pectin, sugars, salts a