The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry
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Genes & Genomics https://doi.org/10.1007/s13258-020-01011-w
RESEARCH ARTICLE
The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry Xiaofan Guo1,2 · Zhibing Xie1,2 · Yang Zhang1,2 · Shouming Wang1,2 Received: 13 August 2020 / Accepted: 13 October 2020 © The Genetics Society of Korea 2020
Abstract Background Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-deficient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’. Objective CYP714C2 was found to be differentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene. Methods In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana. Results The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 significantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modified Arabidopsis lines were significantly higher than that in the wild type. Conclusion We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry. Keywords CYP450 · Gibberellin synthesis · Phytohormone · Wild strawberry · Transformation
Introduction Fruit shape is a major component of overall fruit quality. In strawberries, there is considerable variation in fruit shape that includes conical, globose, and long-wedge shaped fruits determined by the length: width ratio (Rho et al. 2012). Fragaria vesca L., the woodland strawberry, has a small diploid genome (2n = 2x = 14), grows well in cultivation, has a short generation time, and is relatively easy to transform using Agrobacterium tumefaciens (Shulaev et al. 2011). Fragaria vesca fruits are conical in shape. Ethyl methanesulfonate (EMS) was used to mutagenize Fragaria vesca ‘Yellow Wonder’, and a mutant with shorter fruits was identified in the mutant offspring. A detailed characterization of the short-fruit mutant that included analysis of plant phenotype, * Shouming Wang [email protected] 1
School of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China
Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Xiaogan 432000, China
2
pollen viability, genetic characteristics, cytological characteristics, endogenous gibberellic acid (GA) levels, and response to exogenous gibberellic acid application showed it to be a gibberellin-deficient mutant (Wang et al. 2017). Differentially-expressed GA pathway genes were identi
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