The influence of phosphorus source and the nature of nitrogen substrate on the biomass production and lipid accumulation
- PDF / 2,745,897 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 26 Downloads / 152 Views
BIOENERGY AND BIOFUELS
The influence of phosphorus source and the nature of nitrogen substrate on the biomass production and lipid accumulation in oleaginous Mucoromycota fungi Simona Dzurendova 1
1
1
1
2
& Boris Zimmermann & Valeria Tafintseva & Achim Kohler & Dag Ekeberg & Volha Shapaval
1
Received: 22 May 2020 / Revised: 28 July 2020 / Accepted: 5 August 2020 # The Author(s) 2020
Abstract Oleaginous filamentous fungi grown under the nitrogen limitation, accumulate high amounts of lipids in the form of triacylglycerides (TAGs) with fatty acid profiles similar to plant and fish oils. In this study, we investigate the effect of six phosphorus source concentrations combined with two types of nitrogen substrate (yeast extract and ammonium sulphate), on the biomass formation, lipid production, and fatty acid profile for nine oleaginous Mucoromycota fungi. The analysis of fatty acid profiles was performed by gas chromatography with flame ionization detector (GC-FID) and the lipid yield was estimated gravimetrically. Yeast extract could be used as both nitrogen and phosphorus source, without additional inorganic phosphorus supplementation. The use of inorganic nitrogen source (ammonium sulphate) requires strain-specific optimization of phosphorus source amount to obtain optimal lipid production regarding quantity and fatty acid profiles. Lipid production was decreased in ammonium sulphate-based media when phosphorus source was limited in all strains except for Rhizopus stolonifer. High phosphorus source concentration inhibited the growth of Mortierella fungi. The biomass (22 g/L) and lipid (14 g/L) yield of Umbelopsis vinacea was the highest among all the tested strains. Key points • The strain specific P requirements of Mucoromycota depend on the nature of N source. • Yeast extract leads to consistent biomass and lipid yield and fatty acids profiles. • Umbelopsis vinacea showed the highest biomass (22 g/L) and lipid (14 g/L) yield. • High P source amounts inhibit the growth of Mortierella fungi. Keywords Oleaginous fungi . Phosphorus . Nitrogen . Lipid profile . Micro-cultivation
Introduction Unsaturated lipids are essential components in a human and animal nutrition and are traditionally obtained from fish and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10821-7) contains supplementary material, which is available to authorized users. * Simona Dzurendova [email protected]; [email protected] 1
Faculty of Science and Technology, Norwegian University of Life Sciences, Droebakveien 31, 1430 Aas, Norway
2
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Christian Magnus Falsens vei 1, 1433 Aas, Norway
vegetable oils. Monounsaturated lipids are one of the major raw materials for lipid-based biofuels, which are nowadays mostly derived from vegetable and/or waste cooking oils. Recently, increased attention to the ocean protection and fishing regulations for avoiding the overfishing and preser
Data Loading...
