The lncRNA PVT1 promotes invasive growth of lung adenocarcinoma cells by targeting miR-378c to regulate SLC2A1 expressio
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RESEARCH ARTICLE
The lncRNA PVT1 promotes invasive growth of lung adenocarcinoma cells by targeting miR‑378c to regulate SLC2A1 expression Hongwei Xia1 · Zhiqiang Zhang1 · Jun Yuan1 · Qingling Niu2 Received: 14 July 2020 / Accepted: 11 September 2020 © Japan Human Cell Society 2020
Abstract As an oncogene, plasmacytoma variant translocation 1 (PVT1) has been found to be highly expressed in several cancers. However, its specific role in lung adenocarcinoma (ADC) has not been fully elucidated. In this study, the expression of PVT1, miR-378c, and solute carrier family 2 member 1 (SLC2A1) was determined by quantitative real-time PCR and western blot. Dual-luciferase reporter assay was used to explore the relationship between PVT1 and miR-378c, as well as miR-378c and SLC2A1. The effects of PVT1 on the lung ADC cells proliferation, invasion, and migration were detected using MTT, wound-healing, and transwell assays. The results revealed that PVT1 was highly expressed in lung ADC cells, and the overexpression of PVT1 promoted the proliferation, migration, and invasion of lung ADC cells. In lung ADC cells, PVT1 negatively regulated miR-378c expression, and miR-378c negatively regulated SLC2A1 expression through binding to its 3′-untranslated coding regions. Knocking down of PVT1 inhibited the abilities of cell proliferation, migration, and invasion, while miR-378c inhibitor or SLC2A1 Vector diminished the effect. Together, silencing PVT1 downregulated SLC2A1 expression via targeting miR-378c, and then repressed lung ADC cells growth, migration, and invasion. Keywords Lung adenocarcinoma · miR-378c · PVT1 · SLC2A1
Introduction Nonsmall cell lung cancer (NSCLC) is one of the most common malignant tumors worldwide, accounting for about 85% of the total number of lung cancer [1]. The most common subtype of NSCLC is lung adenocarcinoma (ADC), which has a low 5-year survival rate [2]. In the recent years, with the continuous exploration of molecular biology research, the molecular mechanism of lung ADC development has been gradually elucidated [3]. Although some progress has been made in the diagnosis and treatment of lung ADC, the mortality rate is still high due to the complexity of lung ADC occurrence and development [4]. Therefore, a better understanding of the molecular mechanism in lung ADC * Qingling Niu [email protected] 1
Department of Thoracic, Qingpu Branch of Zhongshan Hospital Affiliated To Fudan University, Shanghai 201700, China
Department of Peditrict, Qingpu Branch of Zhongshan Hospital Affiliated To Fudan University, No. 1158, East Park Road, Qingpu District, Shanghai 201700, China
2
development, and the discovery of the oncogene markers in lung ADC etiology and metastasis are particularly important for finding new treatments for lung ADC. Long chain noncoding RNAs (lncRNAs) belong to noncoding RNA with a length of more than 200 nucleotides, which can be involved in a series of cell life processes such as proliferation, apoptosis, and differentiation [5]. Many studies have demonstrated that
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