The polyphasic re-identification of a Brazilian Aspergillus section Terrei collection led to the discovery of two new sp
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ORIGINAL ARTICLE
The polyphasic re-identification of a Brazilian Aspergillus section Terrei collection led to the discovery of two new species Ana C. R. Barros Correia 1 & Renan N. Barbosa 1
&
Jens C. Frisvad 2 & Jos Houbraken 3 & Cristina M. Souza-Motta 1
Received: 5 June 2020 / Revised: 9 July 2020 / Accepted: 10 July 2020 # German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Species belonging to Aspergillus section Terrei have a worldwide distribution and are phenotypically similar. Most species grow either moderately or fast on agar media, and their conidial colors are often in shades of cinnamon brown. In the present study, 79 section Terrei strains stored at the University Recife Mycologia (URM) Culture Collection (Micoteca URM) in Brazil were reidentified using a polyphasic approach, combining partial β-tubulin (BenA) and calmodulin (CaM) gene sequences and morphological data. Seven known (A. terreus, A. pseudoterreus, A. alabamensis, A. citrinoterreus, A. hortai, A. neoafricanus, and A. niveus) and two new species, A. barbosae and A. recifensis, were present among the studied strains. The new species were described based on internal transcribed spacer (ITS), BenA, CaM, and RNA polymerase II second largest subunit (RPB2) sequences in combination with morphological features and extrolite profiles; the most important characteristics for distinguishing the new from the accepted section Terrei species are given. Aspergillus recifensis produces the mycotoxin citreoviridin and A. barbosae citrinin. Keywords New taxa . Fungal culture collection . Polyphasic approach . Taxonomy
Introduction Aspergillus section Terrei includes species with columnar conidial heads in shades of buff to brown. The most well-known species of this section is A. terreus, which is a ubiquitous fungus. The number of newly described species within this section has grown in recent years. Currently, 17 species with diverse Ana C. R. Barros Correia and Renan N. Barbosa contributed equally to this work. Section Editor: Roland Kirschner * Renan N. Barbosa [email protected] * Cristina M. Souza-Motta [email protected] 1
Micoteca URM - Departamento de Micologia Prof. Chaves Batista, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s/n, Centro de Biociências, Cidade Universitaria, Recife, PE CEP: 50670-901, Brazil
2
Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kongens Lyngby, Denmark
3
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
ecological and morphological features are recognized (Samson et al. 2011; Guinea et al. 2015; Arzanlou et al. 2016; Zhang et al. 2018; Visagie and Houbraken 2020; Huang and Jiang 2020; Houbraken et al. 2020). Strains are frequently isolated from soil, compost heaps, and clinical samples and are contaminants of plant products including stored corn, barley, and peanuts (Kozakiewicz 1989; Oliveira et al. 2013; Guinea et al. 2015; Barbosa et al. 2016, 2020). Some species of
