The role of gonadotrophins in gonocyte transformation during minipuberty
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ORIGINAL ARTICLE
The role of gonadotrophins in gonocyte transformation during minipuberty Emily Burton1,2 · Sanduni Amaya Abeydeera1 · Gulcan Sarila1 · Hyun‑Jung Cho3 · Shengde Wu1,4 · Melissa Yixin Tien1,2 · John Hutson1,2,5 · Ruili Li1,2 Accepted: 27 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Purpose Postnatal surge of gonadotrophins, Luteinizing hormone (LH) and Follicle-Stimulating hormone (FSH) known as minipuberty, is critical for gonocyte maturation into spermatogonial stem cells (SSC) in the testis. Gonadotrophins are essential for optimum fertility in men, but very little is known how they regulate germ cells during minipuberty. This study examined whether gonadotrophins play a role on gonocyte transformation in vivo. Methods Testes from hypogonadal (hpg) mice and their wild type (WT) littermates (n = 6/group) were weighed, and processed in paraffin at postnatal days (D) 0, 3, 6 and 9. Mouse VASA homologue (germ cell marker), anti-Müllerian hormone (Sertoli cell marker) antibodies and DAPI (nuclei marker) were used for immunofluorescence followed by confocal imaging. Germ cells on or off basement membrane (BM) and Sertoli cells/tubule were counted using Image J and analyzed with GraphPad. Results Comparing to WT littermates, there were significantly fewer germ cells on BM/tubule (p
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