The UMAG_00031 gene from Ustilago maydis encodes a putative membrane protein involved in pH control and morphogenesis
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ORIGINAL PAPER
The UMAG_00031 gene from Ustilago maydis encodes a putative membrane protein involved in pH control and morphogenesis Juan Antonio Cervantes‑Montelongo2 · Guillermo Antonio Silva‑Martínez3 · Raquel Pliego‑Arreaga4 · Lorenzo Guevara‑Olvera2 · José Ruiz‑Herrera1 Received: 25 February 2019 / Revised: 18 March 2020 / Accepted: 4 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract We report the characterization of the gene UMAG_00031 from Ustilago maydis, previously identified as upregulated at alkaline pH. This gene is located on chromosome 1 and contains an ORF of 1539 bp that encodes a putative protein of 512 amino acids with an MW of 54.8 kDa. The protein is predicted to contain seven transmembrane domains (TMDs) and a signal peptide suggesting that is located in the cell membrane. Null ΔUMAG_00031 mutants were constructed, and their phenotype was analyzed. The mutant displayed a pleiotropic phenotype suggesting its participation in processes of alkaline pH adaptation independent of the Pal/Rim pathway. Also, it was involved in the dimorphic process induced by fatty acids. These results indicate that the protein encoded by the UMAG_00031 gene possibly functions as a receptor of different signals in the cell membrane of the fungus. Keywords Ustilago maydis · pH control · Fatty acids · Dimorphism
Introduction An important characteristic of many pathogenic fungi is the requirement to change their morphology from yeast to mycelium to produce infection, this feature is called dimorphism (Ruiz-Herrera and León-Ramírez 2012). It is known that this dimorphic transition depends on environmental Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01936-6) contains supplementary material, which is available to authorized users.
and host signals that must be recognized by specific receptors at a cell-membrane level that communicate internally with different signaling pathways (Davis 2003; MartínezEspinoza et al. 2004; Peñalva and Arst 2004). The basidiomycete phytopathogenic fungus Ustilago maydis naturally undergoes a dimorphic transition from yeast to filamentous form to infect the maize plants and complete its life cycle (Banuett and Herskowitz 1989; Bolker et al. 1992; Hartmann et al. 1996). In vitro, this transition is induced by the presence of fatty acids, as the only carbon source in the medium (Klose et al. 2004), by growing the fungus in acidic minimal media with an optimum of pH 3 (Ruiz-Herrera et al. 1995)
* José Ruiz‑Herrera [email protected] Juan Antonio Cervantes‑Montelongo [email protected] Guillermo Antonio Silva‑Martínez [email protected] Raquel Pliego‑Arreaga [email protected] Lorenzo Guevara‑Olvera [email protected] 1
Instituto Politécnico Nacional, Apartado Postal 629, 36500 Irapuato, Gto, Mexico 2
Laboratorio de Biología Molecular, Departamento de Ingeniería Bioquímica, Tecnológico Nacional de México en Celaya, Ave. Tecnológico y An
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