Transcriptional response of Desulfovibrio vulgaris Hildenborough to oxidative stress mimicking environmental conditions
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ORIGINAL PAPER
Transcriptional response of Desulfovibrio vulgaris Hildenborough to oxidative stress mimicking environmental conditions Patrícia M. Pereira · Qiang He · António V. Xavier · Jizhong Zhou · Inês A. C. Pereira · Ricardo O. Louro
Received: 19 September 2007 / Revised: 16 November 2007 / Accepted: 20 November 2007 / Published online: 5 December 2007 © Springer-Verlag 2007
Abstract Sulfate-reducing bacteria (SRB) are anaerobes readily found in oxic–anoxic interfaces. Multiple defense pathways against oxidative conditions were identiWed in these organisms and proposed to be diVerentially expressed under diVerent concentrations of oxygen, contributing to their ability to survive oxic conditions. In this study, Desulfovibrio vulgaris Hildenborough cells were exposed to the highest concentration of oxygen that SRB are likely to encounter in natural habitats, and the global transcriptomic response was determined. Three hundred and seven genes were responsive, with cellular roles in energy metabolism, protein fate, cell envelope and regulatory functions, including multiple genes encoding heat shock proteins, peptidases and proteins with heat shock promoters. Of the
Communicated by Friedrich Widdel. António V. Xavier—deceased May 2006. Electronic supplementary material The online version of this article (doi:10.1007/s00203-007-0335-5) contains supplementary material, which is available to authorized users. P. M. Pereira · A. V. Xavier · I. A. C. Pereira · R. O. Louro (&) Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Lisboa, Portugal e-mail: [email protected] Q. He · J. Zhou Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA Q. He · J. Zhou Virtual Institute for Microbial Stress and Survival, Berkeley, CA 94720, USA
oxygen reducing mechanisms of D. vulgaris only the periplasmic hydrogen-dependent mechanism was up-regulated, involving the [NiFeSe] hydrogenase, formate dehydrogenase(s) and the Hmc membrane complex. The oxidative defense response concentrated on damage repair by metalfree enzymes. These data, together with the down-regulation of the ferric uptake regulator operon, which restricts the availability of iron, and the lack of response of the peroxide-sensing regulator operon, suggest that a major eVect of this oxygen stress is the inactivation and/or degradation of multiple metalloproteins present in D. vulgaris as a consequence of oxidative damage to their metal clusters. Keywords Oxidative stress · Desulfovibrio · Genomics · Metalloproteins · Fur · PerR · Thioredoxin · Hmc Abbreviations D Desulfovibrio PMF Proton motive force ROS Reactive oxygen species SRB Sulfate-reducing bacteria
Present Address: P. M. Pereira Departamento de Biologia, Universidade de Aveiro, 3810-193 Aveiro, Portugal Present Address: Q. He Department of Civil and Environmental Engineering, The University of Tennessee, Knoxville, TN 37996, USA Present Address: J. Zhou Department of Botany and Microbiology, Institute for Environmental Genomics, University of Oklahom
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