Transcriptional response of Pseudomonas chenduensis strain MBR to cadmium toxicity
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GENOMICS, TRANSCRIPTOMICS, PROTEOMICS
Transcriptional response of Pseudomonas chenduensis strain MBR to cadmium toxicity Lingjuan Li 1,2 & Qiang Lin 3 & Tiezhu Li 1 & Xiaohong He 1 & Shuming Peng 4 & Yong Tao 1 Received: 26 July 2020 / Revised: 16 September 2020 / Accepted: 21 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Cadmium (Cd) contamination has resulted in serious environmental pollution and threatens human health and ecosystems. Our recent studies have demonstrated that Pseudomonas chenduensis strain MBR can decrease Cd bio-availability and reduce Cd accumulation in rice; however, the transcriptional mechanisms underlying the bacterial response during and particularly after Cd bioremediation are still unclear. In this study, we used RNA-Seq to investigate the transcriptional profiles of strain MBR during and after Cd bioremediation. During Cd bioremediation, MBR removed all Cd2+ ions in solution within 24 h, accompanied by 564 upregulated and 363 downregulated expressed genes compared with that of the control (without Cd supplementation). Specifically, under Cd stress, the upregulation of czc (czcA, czcB, and czcC) and mer (merA, merT, merC, and merP) genes enabled Cd efflux from the cytoplasm and conferred resistance of MBR to Cd toxicity. The upregulation of genes (algK, algX, and alg44) related to biofilm formation enabled Cd absorption and contributed to Cd bioremediation. After Cd bioremediation, MBR was transferred to non-Cd medium, and the genes related to histidine metabolism and flagellar assembly still showed similar expression patterns as those during bioremediation (defined as Cd legacy effects). However, the genes involved in Cd resistance and bioremediation were not influenced by Cd legacy effects. This study provides new and thorough insights into the molecular mechanisms underlying Cd bioremediation by a functional microbe. Key points • The upregulation of czc and mer genes is responsible for MBR resistance to Cd. • The upregulation of genes related to biofilm formation contributes to Cd bioremediation. • Cd effects on genes involved in histidine metabolism and flagellar assembly are long-lasting. Keywords RNA-Seq . Pseudomonas chenduensis strain MBR . Cadmium contamination . Legacy effects
Lingjuan Li and Qiang Lin contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-020-10928-x) contains supplementary material, which is available to authorized users. * Yong Tao [email protected] 1
Key Laboratory of Environmental and Applied Microbiology, CAS & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2
Department of Biology, University of Antwerp, 2610 Wilrijk, Belgium
3
Laboratory of Medical Microbiology, Vaccine & Infectious Disease Institute, University of Antwerp, 2610 Wilrijk, Belgium
4
College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059,
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