Tumor-derived exosomes promote angiogenesis via adenosine A 2B receptor signaling
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ORIGINAL ARTICLE
Tumor-derived exosomes promote angiogenesis via adenosine A2B receptor signaling Nils Ludwig1,2 · Saigopalakrishna S. Yerneni3 · Juliana H. Azambuja1,2,4 · Delbert G. Gillespie5 · Elizabeth V. Menshikova5 · Edwin K. Jackson5 · Theresa L. Whiteside1,2,6 Received: 20 January 2020 / Accepted: 11 May 2020 © Springer Nature B.V. 2020
Abstract Rationale One hallmark of tumor-derived exosomes (TEX) is the promotion of cancer progression by stimulating angiogenesis. This study was performed to evaluate the role of adenosine receptors in TEX-induced angiogenesis. Methods TEX produced by UMSCC47 head and neck cancer cell line were isolated by mini size exclusion chromatography (mini-SEC). Enzymatic activity of ectonucleotidases CD39/CD73 carried by TEX was measured by HPLC. Adenosine content of TEX was measured by UPLC–MS/MS. Primary human macrophages were co-incubated with TEX or exosomes derived from the plasma of head and neck cancer patients and their marker expression profile was analyzed by flow cytometry. The macrophage secretome was analyzed by angiogenesis arrays. The in vitro angiogenic potential of TEX was evaluated in endothelial growth studies. Results were validated in vivo using basement membrane extract plug assays in A1R−/−, A2AR−/− and A 2BR−/− rats. Vascularization was analyzed by hemoglobin quantification and immunohistology with vessel and macrophage markers. Results TEX carried enzymatically active CD39/CD73 and adenosine. TEX promoted A2BR-mediated polarization of macrophages toward an M2-like phenotype (p
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