TWIST1 upregulation affects E-cadherin expression in brain metastases
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RESEARCH ARTICLE
TWIST1 upregulation affects E‑cadherin expression in brain metastases P. Brlek1,2 · A. Bukovac1,2 · A. Kafka1,2 · N. Pećina‑Šlaus1,2 Received: 22 June 2020 / Accepted: 5 September 2020 © Federación de Sociedades Españolas de Oncología (FESEO) 2020
Abstract Purpose E-cadherin is a calcium-dependent glycoprotein whose main role is cell–cell adhesion. Its transcriptional repressor TWIST1 is a basic helix–loop–helix (bHLH) protein that participates in gastrulation and formation of mesodermal tissues during embryogenesis. In adult tissues, the high expression of TWIST1 induces the epithelial–mesenchymal transition (EMT)—a process in which cells become motile and able to metastasize. In this paper, we investigated the involvement of E-cadherin and TWIST1 in the carcinogenesis of brain metastases originating from two different primary sites—breast and lung. Methods The localization and expression of E-cadherin and its transcriptional repressor TWIST1 were investigated using a DAB-labeled streptavidin–horseradish peroxidase immunohistochemical reaction and specific monoclonal antibodies against TWIST1 and E-cadherin. Image J software was used for semi-quantitative analysis while H-score served for statistical evaluations. Results Immunohistochemistry showed that the expression of E-cadherin was downregulated in 85.7% of brain metastases, while at the same time, 82.2% of them showed upregulated TWIST1. Statistical analysis confirmed a significant negative correlation between expressions of TWIST1 and E-cadherin (p = 0.001). When the brain metastases expression levels were compared to primary breast tumors in corresponding patients, E-cadherin showed higher expression in primary pairs compared to corresponding metastases. Consistent to its role, TWIST1 was downregulated in all primary tumor samples in comparison to corresponding metastases pairs (p = 0.034). Conclusion This research provides valuable data regarding molecular events involving two EMT key components that could give directions for new possibilities for brain metastases diagnosis and treatment. Keywords Metastasis · Brain · Epithelial–mesenchymal transition · TWIST1 · E-cadherin Abbreviations AKT Protein kinase B APC Adenomatous polyposis coli CTNNB1 Beta-Catenin EGFR Epidermal growth factor receptor HIF1 Hypoxia-inducible factor 1 LEF1 Lymphoid-enhancer factor 1 LLGL2 Lethal Giant Larvae Homolog 2, Scribble Cell Polarity Complex Component * N. Pećina‑Šlaus [email protected] 1
Department of Biology, School of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, Croatia
Laboratory of Neurooncology, Croatian Institute for Brain Research School of Medicine, University of Zagreb, Šalata 12, 10000 Zagreb, Croatia
2
MAPK Mitogen-activated protein kinase mTOR Mammalian target of rapamycin NFKB1 Nuclear Factor Kappa B Subunit 1 RB1 Retinoblastoma 1 SNAI2 Snail Family Transcriptional Repressor 2 STAT3 Signal transducer and activator of transcription 3 TBC1D2B TBC1 Domain Family Member 2B TCF1 T-cell transcription factor 1 TW
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