Two distinctive Rhizobium genospecies nodulating Vicia villosa Roth in alkaline soils of Northwest China
- PDF / 521,786 Bytes
- 13 Pages / 547.087 x 737.008 pts Page_size
- 19 Downloads / 191 Views
REGULAR ARTICLE
Two distinctive Rhizobium genospecies nodulating Vicia villosa Roth in alkaline soils of Northwest China Junjie Zhang & Yimin Shang & Chunzeng Liu & Entao Wang & Wenfeng Chen & Shanshan Peng & Benyin Li & Chen Guo & Pei Hou & Shuo Li & Brigitte Brunel
Received: 21 October 2019 / Accepted: 28 April 2020 # Springer Nature Switzerland AG 2020
Abstract Aims To estimate the relative abundance, diversity and distribution of rhizobia associated with Vicia villosa Roth in soils of Northwest China. Method Rhizobia were trapped with V. villosa Roth plants from soils at seven sites and identified into genotypes by PCR-RFLP of IGS genes. They were further characterized by phylogenetic analyses of 16S rRNA, housekeeping genes (atpD, recA, glnII) and symbiotic
genes (nodC). Soil physicochemical characteristics were recorded and Canonical Correlation Analysis was performed to correlate soil features and the distribution of IGS genotypes. Results 253 rhizobial strains were obtained from soils with pH 7.1–7.9. They were discriminated into 27 IGS types and further affiliated by phylogenetic analyses of 16S rRNA and multilocus sequence analysis (MLSA) into two clusters in Rhizobium. One corresponds to
Responsible Editor: Ulrike Mathesius. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04549-6) contains supplementary material, which is available to authorized users. J. Zhang (*) : Y. Shang : S. Peng : C. Guo : P. Hou : S. Li College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, People’s Republic of China e-mail: [email protected] J. Zhang Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou, Henan Province 450000, People’s Republic of China J. Zhang Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou, Henan Province 450002, People’s Republic of China C. Liu : B. Li Institute of Plant Nutrition and Resource Environment, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450000, People’s Republic of China
E. Wang Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, C.P. 11340 Ciudad de México, Mexico W. Chen State Key Laboratory of Agrobiotechnology, Beijing 100193, China W. Chen College of Biological Sciences and Rhizobium Research Center, China Agricultural University, Beijing 100193, People’s Republic of China C. Guo Research and Innovation Center of Chunlun Group, Fuzhou 350007, People’s Republic of China B. Brunel LSTM, Univ Montpellier, CIRAD, INRAE, Institut Agro, IRD, Montpellier, France
Plant Soil
Rhizobium sophorae covering 228 strains in 25 IGS types and the other includes a novel genospecies containing two IGS types only detected in Shanxi Province, with higher soil pH and intermediate available phosphate and potassium contents. Representative strains of the novel genospecies show a specific phylogenetic nodC lineage, which may reflect differences in symbiosis
Data Loading...