Type 2 Diabetes Methods and Protocols
Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used l
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
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Type 2 Diabetes Methods and Protocols
Edited by
Claire Stocker Clore Laboratory, University of Buckingham, Hunter Street, Buckingham, UK
iv Editor Claire Stocker, Ph.D. Clore Laboratory University of Buckingham Hunter Street Buckingham United Kingdom MK18 1EG [email protected]
ISSN: 1064-3745 e-ISSN: 1940-6029 ISBN: 978-1-934115-15-2 e-ISBN: 978-1-59745-448-3 DOI: 10.1007/978-1-59745-448-3 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009922609 © Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is for-bidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be life threatening and disabling. Over recent years, both clinical and basic researchers have increased our understanding of the mechanisms involved in the progression of diabetes and the relative roles played by both environmental and genetic factors. For example, it is well known that a lifestyle of inactivity and excessive food intake plays an important part in diabetes risk. The purpose of this volume is to provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Specifically, the chapters will cover the commonly described in vivo and in vitro model systems, ultimately leading to an overall view of how cellular dysfunction and degeneration lead to susceptibility and diabetes disease progression. This book is unique in providing a simple explanation of a particular practical technique or model in a short succinct format, thus allowing research scientists and clinicians quick and easy practical information to address a particular question. There are key practical notes at the end of each chapter, as well as numerous helpful tables and figures.
Claire Stocker Buckingham, UK
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Contents Preface ......................................................................................................................... Contributors......................
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