Up-regulation of miR-182 expression in colorectal cancer tissues and its prognostic value
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ORIGINAL ARTICLE
Up-regulation of miR-182 expression in colorectal cancer tissues and its prognostic value Hui Liu & Lutao Du & Zhihua Wen & Yongmei Yang & Juan Li & Lili Wang & Xin Zhang & Yimin Liu & Zhaogang Dong & Wei Li & Guixi Zheng & Chuanxin Wang
Accepted: 25 February 2013 / Published online: 10 March 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract Purpose Accumulating evidences indicate that dysregulated microRNAs (miRNA) are involved in cancer tumorigenesis and progression. In the present study, we evaluated the expression of miR-182 in colorectal cancer and adjacent noncancerous tissues and explored its associations with clinicopathological characteristics and prognosis. Methods Quantitative real-time PCR was used to analyze the expression of miR-182 in 148 pairs of colorectal cancer and adjacent noncancerous tissues. The relationship between miR-182 expression and clinicopathological characteristics in colorectal cancer tissues was estimated using Mann–Whitney U test or Kruskal–Wallis test, as appropriate. We calculated the survival curves and prognostic values of each variable by the Kaplan–Meier method and Cox proportional hazards regression analysis, respectively. Results The expression of miR-182 was found up-regulated in colorectal cancer tissues compared with adjacent noncancerous tissues (p0.05, respectively) (Table 1). Taken together, these results suggested that a higher level of miR-182 expression may be related with colorectal cancer progression.
Expression of miR-182 in colorectal cancer tissues and adjacent noncancerous tissues
Correlation between miR-182 levels and patients’ survival
The expression levels of miR-182 in 148 paired human colorectal cancer tissues and adjacent noncancerous tissues were quantified by real-time PCR method using LightCycler FastStart DNA Master SYBR Green I (Roche Diagnostic, Mannheim, Germany). Results showed that there was a large variability in the expression of miR-182 across the tissues, with a median value of 0.147 (interquartile range, 0.075–0.277) in colorectal cancer tissues, and a median value of 0.051 (interquartile range, 0.019–0.117) in adjacent noncancerous tissues. Remarkably, we found that miR-182 was up-regulated in colorectal cancer tissues compared with adjacent noncancerous tissues (Fig. 1a, p
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