Using circulating tumor cells to evaluate the efficacy of irreversible electroporation for unresectasble pancreatic canc
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ORIGINAL ARTICLE
Using circulating tumor cells to evaluate the efficacy of irreversible electroporation for unresectasble pancreatic cancer Mao Lin 1,2 & Mohammed Alnaggar 1,3 & Shu-Zhen Liang 1 & Jian Shi 4 & Li-Zhi Niu 1,2,3 & Ji-Bing Chen 1,2 & Ke-Cheng Xu 1,2
# Springer Science+Business Media, LLC, part of Springer Nature 2017
Abstract We used circulating tumor cells (CTCs) as biomarkers to evaluate the efficacy of pre-irreversible electroporation (IRE) and post-IRE for unresectable pancreatic cancer (PC). Real-time qPCR was used to detect potential biomarker genes in CTCs, and magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS) were performed on 43 patients with PC who underwent IRE. Some patients experienced adverse reactions within 30 days of the operation, including arrhythmia (6.9%), intraoperative transient change of blood pressure (25.5%), cough (11.6%), nausea and vomiting (23.3%), ascites (25.6%), fever (9.3%), and pain of puncture point (60.5%). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTCrelated genes CEA, Ep-CAM, and CK19 increased significantly after IRE. Furthermore, the expression of CEA, EpCAM, and CK19 decreased significantly with time after IRE (P < 0.01). Detecting CTCs by RT-qPCR and FACS combined with MACS has significant diagnostic and
Mao Lin and Mohammed Alnaggar shared the first authorship. * Li-Zhi Niu [email protected] * Ji-Bing Chen [email protected]
1
Department of Biological Treatment Center, Fuda Cancer Hospital, Jinan University, Jinan, China
2
Fuda Cancer Institute, Guangzhou, China
3
Department of Oncology, Fuda Cancer Hospital, Jinan University, Jinan, China
4
Department of General Surgery, The Second Hospital of Jilin University, Changchun, Jilin, China
prognostic value for evaluating the efficacy of IRE in patients with unresectable PC. Keywords Circulating tumor cells . Unresectable pancreatic cancer . Real-time quantitative PCR . Biomarker . Efficacy
Introduction Pancreatic cancer (PC) is highly malignant; over 80% of patients are initially diagnosed with unresectable disease [1]. Various ablation methods play important roles in the treatment of locally advanced PC (LAPC). Despite recent advances in surgery, chemotherapy, and radiotherapy, there has been little improvement in the survival of PC [2], and PC continues to be the fourth leading cause of cancer mortality in both men and women. The estimated rates of 1- and 5-year survival of PC are 24 and 4.3%, respectively [3]. Chemotherapy and radiotherapy have been adopted as basic postoperative treatment strategies in metastatic PC; however, their effect is limited and their efficiency appears to have plateaued in the past decade [4–6]. Therefore, identifying more effective therapies for patients with metastatic PC remains an important clinical challenge. Irreversible electroporation (IRE) is an emerging, non-thermal, image-guided tumor ablation technique that has been proven feasible and safe for trea
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