USP10 Expression in Normal Adrenal Gland and Various Adrenal Tumors
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USP10 Expression in Normal Adrenal Gland and Various Adrenal Tumors Zhi Zeng 1 & Ziying Zhou 1 & Na Zhan 1 & Jingping Yuan 1 & Baixin Ye 2 & Lijuan Gu 3 & Jun Wang 4 & Zhihong Jian 4 & Xiaoxing Xiong 3,4
Published online: 10 November 2015 # Springer Science+Business Media New York 2015
Abstract Ubiquitin-specific protease 10 (USP10), a novel deubiquitinating enzyme, is associated with androgen receptor transcriptional activity and pathological processes of tumor. However, information between USP10 and the adrenal gland is limited. In particular, the role of USP10 in adrenal tumors has not been elucidated yet. This study aims to investigate the expression of USP10 in the human normal adrenal gland and various adrenal tumors. Tissue samples were obtained from 30 adrenocortical adenomas, nine adrenocortical adenocarcinomas, and 20 pheochromocytomas following laparoscopic surgery. Twenty normal adrenal glands were obtained from kidney surgical resection conducted due to renal cell carcinomas. USP10 expression was investigated on protein levels using immunohistochemistry and on mRNA levels using bioinformatics analysis in the Gene Expression Omnibus (GEO) Datasets. In the 20 cases of normal adrenal glands analyzed, USP10 protein was constantly expressed in situ in the cortex of the adrenal glands, but in the medulla of the gland, only the sustentacular cells were detected positive. In adrenal tumors, detectable levels of USP10 protein were found in 100 % (30/30)
* Xiaoxing Xiong [email protected] 1
Department of Pathology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People’s Republic of China
2
Department of Hematology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People’s Republic of China
3
Central Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People’s Republic of China
4
Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, People’s Republic of China
adrenocortical adenomas, 88.89 % (8/9) adrenocortical carcinomas, and 10 % (2/20) pheochromocytomas. Bioinformatics analysis did not show a significant difference in USP10 messenger RNA (mRNA) expression between adrenal tumors and normal adrenal gland tissues. A positive USP10 immunoreaction can be useful in distinguishing adrenal cortical tumors from pheochromocytoma. Keywords USP10 . Adrenal gland . Adrenocortical adenoma . Adrenocortical carcinoma . Pheochromocytoma
Introduction Ubiquitin-specific protease 10 (USP10), also known as UBPO, localized on chromosome 16q24.1, and originally discovered as a deubiquitinating enzyme that interacts with Ras-GAP SH3 domain-binding protein (G3BP) [1], takes part in various biological processes by stabilizing several proteins [2, 3]. Increased USP10 expression has been detected in some breast cancer and glioblastoma samples [4], and USP10 overexpression has been associated with poor prognosis in patients with glioblastoma multiforme [5]. Decreased USP10 expression has been observed in gastric cancer tissues, and its downregulation h
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