Utilization of plant-derived Myricetin molecule coupled with ultrasound for the synthesis of gold nanoparticles against
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ORIGINAL ARTICLE
Utilization of plant-derived Myricetin molecule coupled with ultrasound for the synthesis of gold nanoparticles against breast cancer Uma Priya Mohan 1 & Bhaskaran Sriram 2 & Theivendren Panneerselvam 3 & Sankarganesh Devaraj 1 & Davoodbasha MubarakAli 2,4 & Pavadai Parasuraman 5 & Ponnusamy Palanisamy 6 & Adhvitha Premanand 1 & Sankarganesh Arunachalam 1 & Selvaraj Kunjiappan 1 Received: 28 December 2019 / Accepted: 14 April 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Phytochemical mediated synthesis of nanoparticles has gained great interest in the field of cancer therapeutics. We attempted a simple and stable synthesis of gold nanoparticles (AuNPs) with Myricetin (Myr) adopting ultrasound-assisted method. Further, we evaluated anticancer activity of the synthesized nanoparticles. The physico-chemical properties of biosynthesized MyrAuNPs were characterized by UV-visible spectrophotometer, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and particle size analysis. The study reports of Myr-AuNPs showed spherical-shaped particles with a size of < 50 nm. Stability of the particles was increased in various physiological media. Furthermore, the graph theoretical network analysis of Myr-AuNPs indicated that the probable binding with the mTOR is an effective target for breast cancer cells. In silico molecular docking study of Myr-AuNPs in human mTOR kinase was found to be strong binding. The IC50 value of Myr-AuNPs was calculated as 13 μg mL−1 against MCF-7 cell line. The AO/EB and DAPI stainings confirmed the anticancer activity by Myr-AuNPs-treated cells showed a good proportion of dead cells evidenced with formation of pro-apoptotic bodies. In addition, Myr-AuNPs exhibited depolarization of mitochondrial membrane potential and production of reactive oxygen species. This study proves that Myr-AuNPs holds great promise to use against breast cancer as a potent anticancer drug. Keywords Myricetin . Gold nanoparticles . Apoptosis . Breast cancer . Cytotoxicity . MCF-7 cells
Introduction Breast cancer is one of the leading causes of death in women compared to other cancers (Sheffield 2019) and around 11.6
million new cases of breast cancer and 6.6 million deaths have been reported in 2018 (Bray et al. 2018). Although the primary diagnosis of breast cancer helps to achieve a moderate survival rate, distant metastasis hinders the actual survival rate
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00210-020-01874-6) contains supplementary material, which is available to authorized users. * Sankarganesh Arunachalam [email protected] * Selvaraj Kunjiappan [email protected]
3
Department of Pharmaceutical Chemistry, Saraswathi College of Pharmacy, NH-24, Anwarpur, Pilkhuwa, Hapur, Uttar Pradesh 245304, India
4
Division of Bioengineering, Incheon National University, Incheon, Republic of Korea
1
Department of Biotechnology, Kalasalinga
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