Vaccine Technologies for Veterinary Viral Diseases Methods and Proto
This detailed volume explores the most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large scale protein production systems are considered a
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Alejandro Brun Editor
Vaccine Technologies for Veterinary Viral Diseases Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Vaccine Technologies for Veterinary Viral Diseases Methods and Protocols Edited by
Alejandro Brun CISA-INIA, Valdeolmos, Madrid, Spain
Editor Alejandro Brun CISA-INIA Valdeolmos, Madrid, Spain
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3007-4 ISBN 978-1-4939-3008-1 (eBook) DOI 10.1007/978-1-4939-3008-1 Library of Congress Control Number: 2015949635 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface A great amount of knowledge has been generated in recent years on veterinary vaccine technologies, so presently many different antigen delivery systems are available for vaccine research, in particular against viral diseases. This book was intended to review most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large-scale protein production systems are considered among the different chapters of the book. Our aim has been to facilitate the access to real and well-established protocols to those beginning in this interesting and laborious field as well as to provide basic knowledge when attempting a novel vaccine design or platform. This book, in addition, will provide an updated view of most options available when vaccine development is considered. In spite of the number of alternatives available for vaccine design, few of them often reach mar
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