Viral Expression Vectors
In the past ten years there has been enormous progress in the development of eukaryotic viral vectors. In general, these vectors have been developed for one of three reasons: to achieve high levels of expression of a particular gene product (poxvirus, bac
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Editors R. W. Compans, Birmingham/Alabama' M. Cooper, Birmingham/Alabama' H. Koprowski, Philadelphia I. McConnell, Edinburgh· F. Melchers, Basel V. N ussenzweig, New York' M. Oldstone, La lolla/California . S. Olsnes, Oslo' M. Potter, Bethesda/Maryland' H. Saedler, Cologne' P. K. Vogt, Los Angeles' H. Wagner, Munich' I. Wilson, La lolla/California
Viral Expression Vectors Edited byN. Muzyczka
With 20 Figures and 5 Tables
Springer-Verlag Berlin Heidelberg NewYork London Paris Tokyo Hong Kong Barcelona Budapest
NICHOLAS MUZYCZKA
Ph.D.
Associate Professor Department of Microbiology School of Medicine State University of New York at Stony Brook Stony Brook, N.Y. 11794-8621 USA
ISBN-13 :978-3-642-7561 0-8 e-ISBN-13:978-3-642-75608-5 DOl: 10.1007/978-3-642-75608-5 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.
© Springer-Verlag Berlin Heidelberg 1992 Softcover reprint of the hardcover 1st edition 1992 Library of Congress Catalog Card Number 15·12910 The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publishers cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature. Typesetting: Thomson Press (India) Ltd, New Delhi 23/3020-543210 - Printed on acid-free paper.
Preface
In the past ten years there has been enormous progress in the development of eukaryotic viral vectors. In general, these vectors have been developed for one of three reasons: to achieve high levels of expression of a particular gene product (poxvirus, baculovirus, and adenovirus), to clone eukaryotic genes in combination with functional assays (Epstein-Barr virus), of for use as delivery vehicles for the stable introduction of foreign genes into mammalian cells (retroviruses, Epstein-Barr virus, and adeno-associated virus). Each vector has its strengths and weaknesses that are rooted in the sometimes bewildering strategies that the parent viruses use for propagation. No one of these vectors is appropriate for all of the problems that a molecular biology laboratory is likely to encounter, and few of us are knowledgeable in the molecular virology of all of these viruses. This volume represents an attempt by the authors to assemble a r
