Yin Yang 1-induced LINC00667 up-regulates pyruvate dehydrogenase kinase 1 to promote proliferation, migration and invasi
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RESEARCH ARTICLE
Yin Yang 1‑induced LINC00667 up‑regulates pyruvate dehydrogenase kinase 1 to promote proliferation, migration and invasion of cholangiocarcinoma cells by sponging miR‑200c‑3p Jinglin Li1 · Canghai Guan1 · Zengtao Hu1 · Lang Liu1 · Zhilei Su1 · Pengcheng Kang1 · Xingming Jiang1 · Yunfu Cui1 Received: 9 August 2020 / Accepted: 5 October 2020 © Japan Human Cell Society 2020
Abstract Cholangiocarcinoma (CCA) is one of the most aggressive and lethal malignancies. Long noncoding RNAs (lncRNAs) are being found to play crucial roles in CCA progression. This work aims to investigate the roles of long intergenic non-protein coding RNA 667 (LINC00667) in progression of CCA. RT-qPCR and western blot were applied to detect gene expression. Clinical correlation and survival were analyzed by statistical methods. Overexpression and RNA interference approaches were used to investigate the effects of LINC00667 on CCA cells. Tumor xenograft assay was performed to detect the function of LINC00667 in vivo. Transcriptional regulation and competing endogenous RNA (ceRNA) mechanism were predicted via bioinformatics analysis. ChIP, luciferase reporter, and Ago2 RIP assays further confirmed the predicted results. Our data indicated that LINC00667 was highly expressed in CCA tissues and cells, and transcription factor Yin Yang 1 (YY1) induced LINC00667 expression in CCA cells. Up-regulated LINC00667 was significantly associated with lymph node metastasis, advanced TNM stage, and poor prognosis. Knockdown of LINC00667 suppressed the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of CCA cells, while overexpression of LINC00667 acquired opposite effects. Moreover, knockdown of LINC00667 inhibited tumor growth in vivo. In addition, LINC00667 was demonstrated to function as a ceRNA for miR-200c-3p, and then LINC00667 up-regulated pyruvate dehydrogenase kinase 1 (PDK1) to promote CCA development by inhibiting miR-200c-3p. These findings identified a pivotal role of LINC00667 in tumorigenesis and development of CCA. Targeting the YY1/LINC00667/miR-200c-3p/PDK1 axis may provide a new therapeutic strategy for CCA treatment. Keywords Cholangiocarcinoma · LncRNA · LINC00667 · miR-200c-3p · PDK1
Introduction
Jinglin Li, Canghai Guan, and Zengtao Hu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13577-020-00448-1) contains supplementary material, which is available to authorized users. * Xingming Jiang [email protected] * Yunfu Cui [email protected] 1
Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, 148 Baojian Street, Harbin 150086, Heilongjiang, China
Cholangiocarcinoma (CCA) is a group of digestive system malignant tumor that occurs at any location along the biliary tree [1]. The incidence of CCA has been rising over the past two decades in most countries, especially in Thailand with the highest incidence of 96 cases per 100,000 [2]. Early diagnosis of CCA is di
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