3D Composites Based on Hydroxyapatite-Chitosan-Polysiloxane as a Biomimetic Scaffold Materials
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1007-S04-49
3D Composites Based on Hydroxyapatite-Chitosan-Polysiloxane as a Biomimetic Scaffold Materials Andrónico Neira-Carrillo, José Ignacio Arias, M. Soledad Fernández, Ranjith Krishna Pai, Claudia Quilodrán C, and José L Arias CIMAT and Faculty of Veterinary and Animal Science, University of Chile, Santa Rosa 11735, La Pintana, Casilla 2 Correo 15, Santiago, 2-15, Chile
ABSTRACT Three-dimensional (3D) composites of hydroxyapatite, phosphorylated and/or nonphosphorylated chitosan and sulphonated methylsiloxane polymer (HA/P-CHI: CHI/ S-MSP) were prepared by solid-liquid phase separation and solvent sublimation method. The HA, P-CHI and S-MSP were synthesized by chemical continuous wet-calcination, phosphorylation reaction and hydrosilylation-sulphonation reactions, respectively. 3D composites were analyzed by scanning electron microscopy (SEM). Swelling ability, stability and formation of HA particles on the composites immersed in simulated body fluid (SBF) were analyzed. Osteoblasts showed high viability when cultured on composite scaffolds. 3D composites were not degraded after 8 weeks of subcutaneous implantation in rats. INTRODUCTION Improvement of hard composite materials for bone grafting is a continuous challenge for materials technology and biomedical engineering. Biomimetic approaches inspired in biomineralized structures have been explored for development novel biomaterials (1,2). In this context, the use of polymers as templates for the crystallization of inorganic minerals, e.g. CaCO3 and HA, seems to be a promising approach for getting new composite biomaterials (3-6). In previous reports we have described the synthesis of S-MSP and P-CHI functionalized polymers using simple chemical routes and the preparation of synthetically HA particles by chemical continuous wet - calcination method (7). We showed that specific crystallographic of CaCO3 and HA crystals can be controlled by using gas diffusion and double ions diffusion technique, respectively (7). For medical applications, properties such as biocompatibility, nontoxicity, surface activity in controlling crystal nucleation and growth, are desirable. CHI has been developed in different forms for biomedical purposed such as: tubes, coating, spheres, sponges, wound dressings and membranes. The ability to modify the chemical structure of the CHI and polymethylsiloxane (MSP) with different chemical groups and multifunctional molecules, make these polymers good candidates for medical and tissue engineering applications. Based on a biomimetic approach, we have prepared 3D (HA/P-CHI: CHI/ S-MSP) composites from HA, CHI, MSP components using a solid-liquid phase separation and solvent sublimation method. Similar network composite of HA, CHI and cross-linked gel with glutaraldehyde has been prepared elsewhere (8). Biomimetic scaffold materials using HA with collagen provide a good substrate for osteogenic cell (9). HA has been shown to stimulate osteoconduction and is a material that can be integrated into bone with good immunotolerance. Therefore, it h
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