A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and
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BioMed Central
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A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells Luís H Franco1, Pryscilla F Wowk1, Célio L Silva1, Ana PF Trombone1, Arlete AM Coelho-Castelo1, Constance Oliver3, Maria C Jamur3, Edson L Moretto2 and Vânia LD Bonato*1 Address: 1Núcleo de Pesquisas em Tuberculose, Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brasil, 2Laboratório de Fracionamento e Estoque – Centro Regional de Hemoterapia do Hospital das Clínicas, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Rua Tenente Catão Roxo 2501, Ribeirão Preto, SP, Brasil and 3Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo. Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brasil Email: Luís H Franco - [email protected]; Pryscilla F Wowk - [email protected]; Célio L Silva - [email protected]; Ana PF Trombone - [email protected]; Arlete AM Coelho-Castelo - [email protected]; Constance Oliver - [email protected]; Maria C Jamur - [email protected]; Edson L Moretto - [email protected]; Vânia LD Bonato* - [email protected] * Corresponding author
Published: 21 January 2008 Genetic Vaccines and Therapy 2008, 6:3
doi:10.1186/1479-0556-6-3
Received: 27 July 2007 Accepted: 21 January 2008
This article is available from: http://www.gvt-journal.com/content/6/1/3 © 2008 Franco et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: A number of reports have demonstrated that rodents immunized with DNA vaccines can produce antibodies and cellular immune responses presenting a long-lasting protective immunity. These findings have attracted considerable interest in the field of DNA vaccination. We have previously described the prophylactic and therapeutic effects of a DNA vaccine encoding the Mycobacterium leprae 65 kDa heat shock protein (DNA-HSP65) in a murine model of tuberculosis. As DNA vaccines are often less effective in humans, we aimed to find out how the DNA-HSP65 stimulates human immune responses. Methods: To address this question, we analysed the activation of both human macrophages and dendritic cells (DCs) cultured with DNA-HSP65. Then, these cells stimulated with the DNA vaccine were evaluated regarding the expression of surface markers, cytokine production and microbicidal activity. Results: It was observed that DCs and macrophages presented different ability to uptake DNA vaccine. Under DNA stimulation, macrophages, characterized as CD11b+/CD86+/HLA-DR+, produced high levels of TNF-alpha, IL-6 (pro-inflammat
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