A hyperaccumulator plant Sedum alfredii recruits Cd/Zn-tolerant but not Pb-tolerant endospheric bacterial communities fr
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A hyperaccumulator plant Sedum alfredii recruits Cd/ Zn-tolerant but not Pb-tolerant endospheric bacterial communities from its rhizospheric soil Yingjie Wu & Luyao Ma & Xincheng Zhang & Olivera Topalović & Qizhen Liu & Ying Feng & Xiaoe Yang
Received: 26 May 2020 / Accepted: 17 August 2020 # Springer Nature Switzerland AG 2020
Abstract Aims For a metal hyperaccumulator plant Sedum alfredii, the recruitment of unique rhizospheric bacterial communities from bulk soils has been well studied. However, in the root-soil interface, the knowledge on the establishment of endospheric microbiomes from rhizospheric soil is still scarce. Methods In this study, we combined cultureindependent that was 16S rRNA gene amplicon sequencing, and culture-dependent methods that included bacterial isolation, heavy metal tolerance and plant growth-promoting traits. Results The Cd/Zn concentrations in endosphere were significantly higher than in soil, while Pb concentration in endosphere was significantly lower than in soil. The α-diversity in rhizosphere soils was higher than in root endosphere, and the compartments as a major
determinant revealed 85.9% of the taxa variations. The relative abundance of Proteobacteria increased in endosphere compared to rhizosphere. The difference of Cd/Zn tolerance between endospheric and rhizospheric isolates was not obvious, while the Pb tolerance of endospheric isolates significantly decreased compared to rhizosphere. Conclusions The results suggest that S. alfredii recruits Cd/Zn-tolerant but not Pb-tolerant endospheric bacterial communities from its rhizospheric soil. The difference in the microbial structure and function in the root-soil interface might be related to the selective absorption of metals in S. alfredii. Keywords Hyperaccumlator . Phytoremediation . Bacterial community . Heavy metals . Rhizosphere . Endophytes
Responsible Editor: Antony Van der Ent. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04684-0) contains supplementary material, which is available to authorized users. Y. Wu : L. Ma : X. Zhang : Q. Liu : Y. Feng (*) : X. Yang Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road, No. 866, Hangzhou 310058, People’s Republic of China e-mail: [email protected] Y. Wu : O. Topalović Department of Agroecology, Faculty of Technical Sciences, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark
Introduction Plants harbor a high diversity of microorganisms that are associated with various plant habitats, including the rhizosphere, rhizoplane, endosphere and phyllosphere (Zhang et al. 2017; Bai et al. 2015; Cernava et al. 2019). These microorganisms, which mostly include bacteria, archaea, and fungi, are collectively known as plant microbiomes (Turner et al. 2013; Veach et al. 2019; Durán et al. 2018). The plant microbiomes have a profound impact on plant health and growth by providing key fu
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