A NEW THREE-DIMENSIONAL Co(II)-BASED COORDINATION POLYMER: TREATMENT ACTIVITY IN OBESITY MODEL IN VITRO AND IN VIVO BY I
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A NEW THREE-DIMENSIONAL Co(II)-BASED COORDINATION POLYMER: TREATMENT ACTIVITY IN OBESITY MODEL IN VITRO AND IN VIVO BY INCREASING THE LEVEL OF LEPTIN S.-Z. Cui1, Q.-D. Ye1, Y. Chen1, D.-Q. Mao1, Q.-Y. Dai1, N. Li1, and G. Xiao1*
A new 3-dimensional (3D) Co(II)-based coordination polymer, namely, {[Co(L1)](H2O)}n(H2O) (1) (H2L1 = 3,3′-(pyridine-3,5-diyl) dibenzoic acid), is synthesized under the solvothermal reaction conditions. Furthermore, a green-hand grinding technique is implemented to reduce the particle size of complex 1 to generate nanoscale 1 (denoted as nano-1 hereafter). Furthermore, the biological function of the compound is evaluated on the obesity model and the related mechanism is explored. RT-PCR is performed for the evaluation of the leptin gene expression in adipose cells after treating with the compound. ELISA is conducted to determine the leptin content in adipose tissues in vivo after treatment with the compound. DOI: 10.1134/S0022476620070185 Keywords: Co(II)-complex, 3D framework, nanoscale compound, obesity model.
INTRODUCTION The incidence of simple obesity in children has increased significantly in recent years, which has attracted attention of more and more researchers [1]. 80% of children simple obesity will transition to adult obesity. Obesity, hyperinsulinemia, insulin resistance, hyperlipidemia, etc. constitute a metabolic syndrome with an increased risk of cardiovascular disease [2]. The leptin protein is involved in energy metabolism and body weight regulation in mammals as well as in humans, and is involved in a homeostatic regulation mechanism that maintains a constant body fat content [3]. Decreased synthesis of leptin or insensitivity to leptin response may all lead to obesity. However, there are few reports on the candidate compounds which could change the serum leptin level in children with simple obesity [4, 5]. Thus, in this experiment we aimed at developing new candidates for the obesity treatment by targeting leptin level regulation. To date, much efforts have been devoted to the design and preparation of transition metal coordination polymer materials because these metals are physiologically essential metal elements that function in endogenous oxidative DNA damage associated with aging and cancer [6-8]. Metal ions that are essential for cells may become toxic at high concentrations because they can replace other important metals in enzymes [9]. Accordingly, many publications focused on coordination compound materials because of their good biocompatibility. Besides, coordination polymers can interact with DNA through noncovalent interactions, including intercalation and groove binding for large molecules and slot external
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Department of Paediatrics, Ninbo First Hospital, Ningbo, Zhejiang, P. R. China; *[email protected]. Original article submitted September 8, 2019; revised October 14, 2019; accepted October 14, 2019. 1138
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electrostatic binding for cations [10-12]. On the other hand, cobalt is an element of
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