A novel type of cosavirus from children with nonpolio acute flaccid paralysis
- PDF / 560,846 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 48 Downloads / 141 Views
SHORT REPORT
Open Access
A novel type of cosavirus from children with nonpolio acute flaccid paralysis Yan Yang1*, Aiping Ju2, Xiaofen Xu1, Xinyu Cao1 and Ying Tao1
Abstract Human cosavirus (HCoSV) is a genus recently identified in the family Picornaviridae, which contains important pathogens to human health. Here, a novel type of HCoSV strain, cosavirus-zj-1 (GenBank no. KX545380), was identified in the fecal sample of a child with nonpolio acute flaccid paralysis (AFP) in China. Phylogenetic and sequence analyses suggested that this virus strain belonged to a new genotype in HCoSV B species. Our data show that surveillance of HCoSV is necessary for detecting viral agents in children with AFP, despite being the low detection rate. Keywords: Human cosavirus, Complete genome, Phylogenetic analysis
Findings Human Cosavirus (HCoSV) is a new member in the Picornaviridae family. It was originally detected from fecal samples of both healthy children and non-polio acute flaccid paralysis (AFP) patients in Pakistan and Afghanistan as well as in a fecal sample of a 64-year- old woman from Scotland [1, 2]. Subsequently, many studies on HCoSV detection in children and adults in different countries were reported [3–9]. Besides the apparently wide geographic distribution, HCoSV has a wide genetic diversity. The genome of HCoSV is about 7.6 kb long organized in a typical picornavirus genome, the only difference is the absence of the leader (L) sequence [1]. The genome encodes four structural viral proteins (VP4, VP2, VP3, and VP1), and nine nonstructural proteins (2A1, 2A2, 2B, 2C, 3A, 3B1, 3B2, 3C, and 3D) [1]. Based on the VP1 sequences, the genus is currently divided into six genetically distinct species (A-F) including more than 30 genotypes [10]. Similar to cardioviruses, which are close relatives of picornaviruses, HCoSV was frequently detected in the feces of symptomatic as well as asymptomatic subjects, and thus their role in human enteric disease remains unclear. This is partly because there have been only a
* Correspondence: [email protected] 1 The Fourth Affiliated Hospital of Jiangsu University, 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China Full list of author information is available at the end of the article
limited number of epidemiological studies on this emerging virus. In the present study, a total of 29 fecal samples were collected from 29 children between January 2010 and December 2014 with clinical manifestations of AFP but no detection of poliovirus. RT-PCR method was used to amplify a 316-nucleotides fragment of the 5′ non-coding region of the HCoSV genome [1]. Of the 29 fecal samples investigated, only one was positive for HCoSV. The PCR products were T-A cloned, sequenced and then compared to the NCBI nucleotide collection using BLASTn. This revealed that the 316 nt HCoSV fragment shared highest sequence identity (86 %) with a viral sequence belonging to HCoSV A species (HCoSV-A1, FJ438902). In order to get the full genome of this divergent HCoSV strain and investigate whether this fecal
Data Loading...
