A Study of Protein Fractional Composition during Incubation of Diphyllobothrium dendriticum (Cestoda) Plerocercoids in a
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A Study of Protein Fractional Composition during Incubation of Diphyllobothrium dendriticum (Cestoda) Plerocercoids in a Medium Containing Blood Serum of the Host, the Baikal Omul Coregonus migratorius (Coregonidae) I. A. Kutyreva, *, O. B. Gorevab, O. E. Mazura, and V. A. Mordvinovc aInstitute b
of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, Ulan-Ude, 670047 Russia Institute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, 630117 Russia c Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia *e-mail: [email protected] Received May 3, 2018; revised December 20, 2018; accepted March 25, 2019
Abstract—The change in the composition of the protein fraction of Diphyllobothrium dendriticum (Cestoda) plerocercoids during incubation in a medium containing blood serum of their host—the Baikal omul Coregonus migratorius (Coregonidae)—was investigated. The change in the composition of the protein fraction of the incubation medium also was studied. On the one hand, the appearance of new high-molecular fractions with a molecular weight (MW) of 193 kDa after 3, 12, and 24 h of incubation and 88 kDa after 24 h was detected, which was associated at plerocercoids with the appearance of a new 189 kDa fraction after 12 h and the disappearance of the 80 kDa fraction. On the other hand, a decrease or the disappearance of two low-molecular fractions 57 and 42 kDa in the incubation medium after 3, 12, and 24 h of incubation were observed. DOI: 10.1134/S1062359020040081
INTRODUCTION Parasitism is one of the most successful lifestyles practiced by living organisms, given the frequency of its occurrence and the huge number of parasitic species (Poulin and Morand, 2000). Numerous defense mechanisms against the immune response of the host allow parasites to grow, survive, and persist for a long time in the host (Maizels et al., 2004; Coakley et al., 2016). Parasites regulate the immune response of their hosts by secretion of soluble mediators that interact with cells and molecules of the immune system in a certain way (Lightowlers and Rickard, 1988). In recent years, interest has increased in identifying immunoregulatory molecules produced by parasitic worms. Parasitic immunoregulators are diverse both in their functional activity and in their chemical nature. The most important group of immunoregulatory substances of parasites is proteins. These include cytokine homologs, protease inhibitors, glycolytic enzymes and lectins, antioxidants, acetylcholinesterase, and poison allergen homologues (VAL), among others (Jenkins et al., 2005; Hewitson et al., 2009; Lvova et al., 2014). Therefore, study of the proteins of the secretory– excretory products of parasites produced in response to interaction with the host tissues is of interest. At
present, the immunoregulatory molecules of fish parasites remain poorly understood. Cestodes in some endemic regions by their epidemiological and ep
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