A TLC-HPLC Method for Determination of Thiamphenicol in Pig, Chicken, and Fish Feedstuffs
- PDF / 921,744 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 65 Downloads / 210 Views
A TLC-HPLC Method for Determination of Thiamphenicol in Pig, Chicken, and Fish Feedstuffs JianMei Wang 1 & JinJing Yang 2 & MingRong Qian 1 & DanNa Zhou 3 & XianBing Ke 2 & GuiZhi Sun 2 & Bo Yang 2 Received: 27 December 2017 / Accepted: 16 May 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018
Abstract An effective thin-layer chromatography (TLC) purification procedure coupled to high-performance liquid chromatography (HPLC) method was developed for the determination of thiamphenicol (TAP) in pig, chicken, and fish feedstuffs. The feedstuff samples were extracted with ethyl acetate, defatted with n-hexane saturated with acetonitrile, and further purified by TLC. The chromatographic separation was performed on a Waters Symmetry C18 column using an isocratic procedure with acetonitrilewater (21.7:78.3, v/v) at 0.6 mL/min. The ultraviolet (UV) detector was set at a wavelength of 225 nm. The TAP concentrations in feedstuff samples were quantified using a standard curve. Good linear correlations (y = 162,630x − 2381.7, r > 0.9998) were achieved within the concentration range of 0.05–10.00 μg/mL. The recoveries of TAP spiked at levels of 1, 10, and 100 μg/g ranged from 82.0 to 114.9% with the intra-day and inter-day relative standard deviation (RSD) less than 9.0%. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.02 and 0.03 mg/kg for pig feedstuffs, 0.02 and 0.04 mg/kg for chicken feedstuffs, and 0.02 and 0.03 mg/kg for fish feedstuffs, respectively. This reliable, simple, and cost-effective method could be applied to the routine monitoring of TAP in animal feedstuffs. Keywords High-performance liquid chromatography . Thin-layer chromatography . Thiamphenicol . Animal feedstuffs
Introduction TAP is a broad-spectrum phenicol antimicrobial agent against both Gram-positive and Gram-negative bacteria as well as other groups of microorganisms. In China, TAP is approved to be administered to fish by incorporation into feedstuffs for the control of bacterial diseases (Ministry of Agriculture Bulletin 2001). The misuse of TAP (such as excessive addition of TAP to fish feedstuffs or illegal incorporation of TAP
into pig and chicken feedstuffs) can cause the presence of its residues in animal-derived foods and induce antibacterial resistance. For consumer protection, the routine monitoring of TAP in animal feedstuffs should be taken, and reliable analytical method is required for this purpose. Several analytical methods have been published for determining TAP in animal feedstuffs based on liquid chromatography-mass spectrometry (LC-MS) (León et al. 2016; Ministry of Agriculture Bulletin 2016) and enzyme-
JianMei Wang and JinJing Yang contributed equally to this work. * Bo Yang [email protected] JianMei Wang [email protected]
GuiZhi Sun [email protected] 1
State Key Laboratory Breeding Base for Zhejiang Sustainable Plant Pest Control; Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People’s Republic of China
2
Hubei
Data Loading...
