Acetylome analysis of the feline small intestine following Toxoplasma gondii infection
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GENETICS, EVOLUTION, AND PHYLOGENY - ORIGINAL PAPER
Acetylome analysis of the feline small intestine following Toxoplasma gondii infection Yu-Meng Meng 1,2 & Bin-Tao Zhai 2 & Hany M. Elsheikha 3 & Shi-Chen Xie 2 & Ze-Xiang Wang 4 & Quan Zhao 1 & Xing-Quan Zhu 1,2,5 & Jun-Jun He 2 Received: 28 May 2020 / Accepted: 7 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Toxoplasma gondii is a protozoan parasite capable of infecting a large number of warm-blooded animals and causes serious health complications in immunocompromised patients. T. gondii infection of the feline small intestine is critical for the completion of the life cycle and transmission of T. gondii. Protein acetylation is an important posttranslational modification, which plays roles in the regulation of various cellular processes. Therefore, understanding of how T. gondii reprograms the protein acetylation status of feline definitive host can help to thwart the production and spread of T. gondii. Here, we used affinity enrichment and high-resolution liquid chromatography with tandem mass spectrometry to profile the alterations of the acetylome in cat small intestine 10 days after infection by T. gondii Prugniuad (Pru) strain. Our analysis showed that T. gondii induced significant changes in the acetylation of proteins in the cat intestine. We identified 2606 unique lysine acetylation sites in 1357 acetylated proteins. The levels of 334 acetylated peptides were downregulated, while the levels of 82 acetylated peptides were increased in the infected small intestine. The proteins with differentially acetylated peptides were particularly enriched in the bioenergeticsrelated processes, such as tricarboxylic acid cycle, oxidative phosphorylation, and oxidation-reduction. These results provide the first baseline of the global acetylome of feline small intestine following T. gondii infection and should facilitate further analysis of the role of acetylated protein in the pathogenesis of T. gondii infection in its definitive host. Keywords Toxoplasma gondii . Posttranslational modification . Cat . Lysine acetylation
Introduction Toxoplasma gondii is an obligate intracellular pathogen that has been estimated to chronically infect approximately one-
third of the world’s population (Dubey 2010). However, T. gondii causes severe diseases in prenatally infected children and in immunocompromised individuals (Hohlfeld et al. 1989; Luft and Remington 1992). Although T. gondii has a
Handling Editor: Julia Walochnik Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00436-020-06880-4) contains supplementary material, which is available to authorized users. * Xing-Quan Zhu [email protected]
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Faculty of Medicine and Health Sciences, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
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College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu Province 730070, People’s Republic of China
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Col
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