Acquired antibody responses against merozoite surface protein-1 19 antigen during Plasmodium falciparum and P.vivax infe
- PDF / 1,298,873 Bytes
- 15 Pages / 595.276 x 790.866 pts Page_size
- 34 Downloads / 176 Views
ORIGINAL ARTICLE
Acquired antibody responses against merozoite surface protein119 antigen during Plasmodium falciparum and P.vivax infections in South Indian city of Mangaluru Kishore Punnath1,2 • Kiran K. Dayanand1,2 • Vishal Midya3 • Valleesha N. Chandrashekar1,2 Rajeshwara N. Achur1 • Srinivas B. Kakkilaya4 • Susanta K. Ghosh5 • Suchetha N. Kumari2 • D. Channe Gowda6
•
Received: 15 May 2020 / Accepted: 5 October 2020 Ó Indian Society for Parasitology 2020
Abstract Merozoite surface protein-1 (MSP-1) of malaria parasites has been extensively studied as a malaria vaccine candidate and the antibody response to this protein is an important indicator of protective immunity to malaria. Mangaluru city and its surrounding areas in southwestern India are endemic to malaria with Plasmodium vivax being the most widespread and prevalent species although P. falciparum also frequently infects. However, no information is available on the level of protective immunity in this
population. In this regard, a prospective hospital-based study was performed in malarial patients to assess antibody responses against the 19-kDa C-terminal portion of P. vivax and P. falciparum MSP-1 (MSP-119). Serum samples from 51 healthy endemic controls and 267 infected individuals were collected and anti-MSP-119 antibody levels were analyzed by ELISA. The possible association between the antibody responses and morbidity parameters such as malarial anemia and thrombocytopenia was
& Rajeshwara N. Achur [email protected]
3
Kishore Punnath [email protected]
Department of Biostatistics and Bioinformatics, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA, USA
4
Kiran K. Dayanand [email protected]
Light House Polyclinic, Light House Hill Road, Mangaluru, Karnataka, India
5
Vishal Midya [email protected]
Department of Molecular Parasitology, ICMR-National Institute of Malaria Research, Poojanahalli, Bangalore, India
6
Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA, USA
Valleesha N. Chandrashekar [email protected] Srinivas B. Kakkilaya [email protected] Susanta K. Ghosh [email protected] Suchetha N. Kumari [email protected] D. Channe Gowda [email protected] 1
Department of Biochemistry, Kuvempu University, Shankaraghatta, Shivamogga District, Karnataka, India
2
Department of Biochemistry, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangaluru, India
123
J Parasit Dis
investigated. Among the 267 infected cases, 144 had P. vivax and 123 had P. falciparum infections. Significant levels of anti-MSP-119 antibody were observed both in P. vivax (123/144; 85.4%) and P. falciparum (108/123; 87.9%) infected individuals. In both type of infections, the major antibody isotypes were IgG1 and IgG3. The IgG levels were found to be increased in patients with severe anemia and thrombocytopenia. The antibody levels were also higher in infected individuals who had
Data Loading...
