Adhesion of indigenous Lactobacillus plantarum to gut extracellular matrix and its physicochemical characterization
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ORIGINAL PAPER
Adhesion of indigenous Lactobacillus plantarum to gut extracellular matrix and its physicochemical characterization Ashok Kumar Yadav · Ashish Tyagi · Ashwani Kumar · Asha Chandola Saklani · Sunita Grover · Virender Kumar Batish
Received: 18 May 2014 / Revised: 24 August 2014 / Accepted: 26 August 2014 © Springer-Verlag Berlin Heidelberg 2014
Abstract Adhesion to the human intestinal epithelial cell is considered as one of the important selection criteria of lactobacilli for probiotic attributes. Sixteen Lactobacillus plantarum strains from human origins were subjected for adhesion to extracellular matrix (ECM) components, and their physiochemical characterization, incubation time course and effect of different pH on bacterial adhesion in vitro were studied. Four strains showed significant binding to both fibronectin and mucin. After pretreatment with pepsin and trypsin, the bacterial adhesion to ECM reduced to the level of 50 % and with lysozyme significantly decreased by 65–70 %. Treatment with LiCl also strongly inhibited (90 %) the bacterial adhesion to ECM. Tested strains showed highest binding efficacy at time course of 120 and 180 min. Additionally, the binding of Lp91 to ECM was highest at pH 6 (155 ± 2.90 CFU/well). This study proved
Communicated by Erko Stackebrandt. A. K. Yadav (*) Department of Microbiology and Immunology, National Institute of Nutrition, Hyderabad, India e-mail: [email protected] A. Tyagi · S. Grover · V. K. Batish (*) Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India e-mail: [email protected] A. Kumar Department of Biotechnology, Seth Jai Parkash Mukand Lal Institute of Engineering and Technology, Radaur, Yamuna Nagar 135133, Haryana, India e-mail: [email protected] A. C. Saklani School of Life Sciences, Appejay Stya University, Gurgaon, Haryana, India
that surface layer components are proteinaceous in nature, which contributed in adhesion of lactobacillus strains. Further, the study can provide a better platform for introduction of new indigenous probiotic strains having strong adhesion potential for future use. Keywords Lactobacillus plantarum · Adhesion · Extracellular matrix · Physicochemical characterization
Introduction Among lactic acid bacteria (LAB), the genus Lactobacilli are commonly used as probiotic organisms, which help to maintain a balanced intestinal microbiota, detoxifying colonic toxins, lowering serum cholesterol levels (Kumar et al. 2011; Grover et al. 2012), promoting lactose tolerance, producing metabolites crucial to the function of intestinal epithelial cells (Szilagyi et al. 2010), excluding pathogens and assisting to keep the gut homeostasis by influencing the mucosal immune system (Kumar et al. 2011; Duary et al. 2012a; Hardy et al. 2013; Yadav et al. 2013). Colonization of a probiotic strain on the mucosal surface is undoubtedly a primary prerogative for stable and successive exertion of these beneficial effects in the gut. Successful colonization i
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