Advanced Flow Cytometry: Applications in Biological Research

Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular

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Edited by

R.

c.

Sobti

Department of Bio-Technology, Panjab University, Chandigarh 160014 India

and

Awtar Krishan Department of Radiation Oncology, University of Miami Medical School, Miami, FL 33101 U.S.A.

Reprinted from Methods in Cell Science, Volume 24(1-3), 2002

SPRINGER-SCIENCE+BUSINESS MEDIA, B.V.

A C.I.P. Catalogue record for this book is available from the Library of Congress.

ISBN 978-90-481-6368-7 ISBN 978-94-017-0623-0 (eBook) DOI 10.1007/978-94-017-0623-0

Printed on acid-free paper

AH Rights Reserved. © 2003 Springer Science+Business Media Dordrecht Originally published by Kluwer Academic Publishers in 2003 Softcover reprint ofthe hardcover Ist edition 2003 No part of the material protected by this copyright notice may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording or by any information storage and retrieval system, without written permission from the copyright owner.

Table of Contents

Flow cytometry, an overview L. Scott Cram

1-9

High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue Richard A. Thomas, Awtar Krishan & Michael Brochu

11-18

Analysis of heterogeneous red cell populations by flow cytometry M. Nelson

19-25

Chromosome sorting and genomics L. Scott Cram, Carolyn S. Bell & John J. Fawcett

27-35

Analysis of intracellular cytokines using flowcytometry Sunil K. Arora

37--40

Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle Tomoko Furuya, Morihito Takita, Shin-ichi Tsunoda, Shigeto Kawauchi, Takashi Hirano, Atsunori Oga & Kohsuke Sasaki

41--47

Flow cytometry and its application in small animal oncology Katja Culmsee & Ingo Nolte

49-54

Flow cytometric monitoring of drug resistance in human tumor cells Awtar Krishan

55-60

Androgen receptor expression in archival human breast tumors Poonam Arya, Ilia H. Andritsch & Awtar Krishan

61-64

Membrane oxidative damage and apoptosis in cervical carcinoma cells of patients after radiation therapy S. M. Bhosle, B. N. Pandey, N. G. Huilgol & K. P. Mishra

65-68

Nitric oxide mediated modulation of free radical generation response in the rat polymorphonuclear leukocytes: A flowcytometric study Madhu Dikshit & Prashant Sharma

69-76

Flowcytometric detection of PNH defect and response to therapy in aplastic anemia patients Neelam Varma, Subhash Varma, Harpreet Vohra, Kiran Malik & Gurjeevan Garewal

77-78

Host-pathogen interactions: leukocyte phagocytosis and associated sequelae Jovanka M. Voyich & Frank R. DeLeo

79-90

Flow cytometric techniques for the detection of microorganisms Hazel M. Davey

91-97

A flow cytometric approach to assess phytoplankton respiration Gerald Gregori, Michel Denis, Dominique Lefevre & Beatriz Beker Cellular and serological markers of disease activity in Indian patients with HIV / AIDS S. Sehgal, U. Datta, S. Mujtaba, A. Sood and V. K. Vinayak

99-106 107-114

Methods in Cell Science 24: 1- 9