Flow Cytometry Protocols
This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellu
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Teresa S. Hawley Robert G. Hawley Editors
Flow Cytometry Protocols Fourth Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Flow Cytometry Protocols Fourth Edition
Edited by
Teresa S. Hawley Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Robert G. Hawley Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, DC, USA
Editors Teresa S. Hawley Flow Cytometry Section Research Technologies Branch National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda, MD, USA
Robert G. Hawley Department of Anatomy and Regenerative Biology School of Medicine and Health Sciences George Washington University Washington, DC, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7344-6 ISBN 978-1-4939-7346-0 (eBook) DOI 10.1007/978-1-4939-7346-0 Library of Congress Control Number: 2017954575 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedicated to our loving parents Wing Han Chan, Sheung Tak Lam, Vietta Ruth Hawley, and George Robert Hawley
Preface In the last edition, we noted that we were riding the crest of remarkable advances in innovative technologies. We did not anticipate that 6 years later we would still be in the midst of groundbreaking innovations that push
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