Flow Cytometry Basics for the Non-Expert

Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has pro

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Christine Goetz  Christopher Hammerbeck  Jody Bonnevier

Flow Cytometry Basics for the Non-Expert

Techniques in Life Science and Biomedicine for the Non-Expert Series editor Alexander E. Kalyuzhny, Bio-Techne, Inc., Minneapolis, MN, USA

The goal of this series is to provide concise but thorough introductory guides to various scientific techniques, aimed at both the non-expert researcher and novice scientist. Each book will highlight the advantages and limitations of the technique being covered, identify the experiments to which the technique is best suited, and include numerous figures to help better illustrate and explain the technique to the reader. Currently, there is an abundance of books and journals offering various scientific techniques to experts, but these resources, written in technical scientific jargon, can be difficult for the non-expert, whether an experienced scientist from a different discipline or a new researcher, to understand and follow. These techniques, however, may in fact be quite useful to the non-expert due to the interdisciplinary nature of numerous disciplines, and the lack of sufficient comprehensible guides to such techniques can and does slow down research and lead to employing inadequate techniques, resulting in inaccurate data. This series sets out to fill the gap in this much needed scientific resource. More information about this series at http://www.springer.com/series/13601

Christine Goetz • Christopher Hammerbeck Jody Bonnevier

Flow Cytometry Basics for the Non-Expert

Christine Goetz Department of Antibody Development R&D Systems/Bio-Techne Minneapolis, MN, USA

Christopher Hammerbeck Department of Antibody Development R&D Systems/Bio-Techne Minneapolis, MN, USA

Jody Bonnevier Department of Antibody Development R&D Systems/Bio-Techne Minneapolis, MN, USA

ISSN 2367-1114     ISSN 2367-1122 (electronic) Techniques in Life Science and Biomedicine for the Non-Expert ISBN 978-3-319-98070-6    ISBN 978-3-319-98071-3 (eBook) https://doi.org/10.1007/978-3-319-98071-3 Library of Congress Control Number: 2018953158 © Springer Nature Switzerland AG 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor