Advanced Protocols in Oxidative Stress II
Expanding upon the research elucidated by the first volume of this collection, Advanced Protocols in Oxidative Stress II presents thirty additional cutting-edge chapters focusing on novel techniques for detecting ROS/RNS, unique AOX technology and applica
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Advanced Protocols in Oxidative Stress II Edited by
Donald Armstrong University at Buffalo, Buffalo, NY, USA University of Florida, Gainesville, FL,USA
Editor Donald Armstrong University at Buffalo Buffalo, NY, USA University of Florida Gainesville, FL,USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-410-4 e-ISBN 978-1-60761-411-1 DOI 10.1007/978-1-60761-411-1 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009930359 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: In vivo multiphoton image of the redox ratio (fluorescence intensity of FAD/NADH) from a 50 micron depth in low grade dysplastic tissue in the hamster cheek pouch. The redox ratio highlights metabolic activity in the electron transport chain within cellular mitochondria. Comparisons between redox ratio images can provide relative measurements of metabolic activities in cells, where a decrease in the redox ratio indicates increased oxidative metabolic activity. Pink pixels represent low redox values and yellow pixels represent high redox values. The image is 100 x 100 microns (figure credit: Melissa Skala). Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface The present volume is the second in the continuation of the new collection of oxidative stress (OS) protocols that focus on novel techniques for detecting ROS/RNS, unique AOX technology and applications, gene expression and biostatistics for evaluating OS-derived experimental data. It presents 30 additional chapters to Volume I. These current methods expand on those in Advanced Protocols I, giving researchers the opportunity to have 61 total
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