Alginate Calcium Microbeads Containing Chitosan Nanoparticles for Controlled Insulin Release
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Alginate Calcium Microbeads Containing Chitosan Nanoparticles for Controlled Insulin Release Jinglei Li 1
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& Haishan Wu & Kexin Jiang & Yuting Liu & Liu Yang & Hyun Jin Park
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Received: 12 November 2019 / Accepted: 11 September 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Effective delivery system for oral insulin administration is a promising way for diabetes therapy. Herein, we prepared alginate microbeads containing chitosan nanoparticles (CNP) for controlled release of insulin. CNP was developed by reaction between tripolyphosphate (TPP) and chitosan. The ratio of TPP to chitosan was optimized aiming with smaller and more unified distributed CNP. TEM and DLS analysis confirmed that CNP has size around 150 nm with low PDI value and strong surface charge. Encapsulate ability for bovine serum albumin, working as model protein, was 11.45%, and the encapsulate efficiency was 23.70%. To modify the release profile of protein suitable for oral insulin delivery, sodium alginate was applied to coat on the surface of CNP by electrostatic interaction. After that, CaCl2 was added to reinforce the alginate coating layer. FTIR analysis confirmed the interaction of alginate with chitosan and reaction with calcium ion. After reaction with Ca2+ ion, size measurement revealed that CNP was incorporated into alginate microbeads with mean diameter about 3.197 μm. Alginate microbeads presented irregular shape with small particles inside as revealed by optical microscope. Meanwhile, the release test demonstrated that protein release was pH-dependent. Acidic pH value retards protein release and neutral pH value promotes protein release. At last, insulin-loaded alginate microbeads were administrated to hyperglycemia model mice and blood glucose profile was monitored afterward. Insulin-loaded microbeads significantly lowered blood glucose level compared with mice treated with alginate microbeads without insulin. It is noted that insulin-loaded alginate microbeads could lower blood glucose level in much prolonged period of 96 h, indicating that insulin was released in controlled manner. Keywords Controlled release . Insulin . pH responsible . Alginate coating . Encapsulation * Jinglei Li [email protected]
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Engineering Research Center of Bioprocess, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009 Anhui, China
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Shaanxi Key Laboratory for Animal Conservation, and School of Life Science, Northwest University, Xi’an, China
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School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea
Applied Biochemistry and Biotechnology
Introduction Insulin administration is the most effective therapy for diabetes patients to control hyperglycemia and other complications [1]. However, usage of insulin is still quite problematic due to its administration method. By far, subcutaneous injection is the commonest way which also generates problems like allergic reaction, possible infection, skin bulges, needle phobia, and low patien
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