Alginate-honey bioinks with improved cell responses for applications as bioprinted tissue engineered constructs
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Downloaded from https://www.cambridge.org/core. Tufts Univ, on 01 Jul 2018 at 13:24:04, subject to the Cambridge Core terms of use, available at https://www.cambridge.org/core/terms. https://doi.org/10.1557/jmr.2018.202
Alginate-honey bioinks with improved cell responses for applications as bioprinted tissue engineered constructs Sudipto Datta, Ripon Sarkar, and Veena Vyas Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Sumant Bhutoria Alfatek Systems, Kolkata-700033, W.B., India
Ananya Barui Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Amit Roy Chowdhury Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India; and Department of Aerospace Engineering and Applied Mechanics, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Pallab Dattaa) Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India (Received 28 March 2018; accepted 1 June 2018)
The polysaccharide alginate has received most extensive attention as bioink in bioprinting applications due to its ability to undergo gelation under cell-friendly conditions. However, absence of cell-binding motifs and the erratic degradation of alginate hydrogels have remained their persistent limitations. Honey is a conveniently available natural material, known for its role in wound healing and skin tissue regeneration. However, honey blending to improve biological response of alginate-based bioprinted scaffolds has not been yet reported. In the present work, honey-alginate bioinks were evaluated for their printability property (shape fidelity). It was found that honey blending reduced alginate viscosity, which gradually affected bioprinting fidelity. Therefore, the concentration that provides for acceptable bioprinting along with improvement in cell proliferations is determined. It is concluded that honey blending improves cell response of alginate bioinks and can be a facile approach to obtain bioinks especially for in situ skin tissue engineering applications.
I. INTRODUCTION
In the last few years, 3D printing has emerged as a promising fabrication method to generate engineered tissue constructs for both in vitro disease modeling and tissue engineering applications.1 Bioprinting has further expanded the domain in which live cells can be deposited according to desired structure, shape, and cell-biomaterial compositions.2 These techniques leverage a computercontrolled device to dispense biomaterials and/or cells into accurate, anatomically complex structures.3,4 Although several technology platforms are now available for fabricating tissue constructs by bioprinting, extrusionbased bioprinting has emerged as a conveniently scalable, versatile, and affordable technique to generate mechanically rob
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