Silk fibroin sponge combined with cell-derived ECM for tissue-engineered 3D functional neural tissues
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lk fibroin sponge combined with cell-derived ECM for tissueengineered 3D functional neural tissues 1
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ZHU HuiMin , LIU Wei , YIN Qi , LI SiWei , WANG ChangYong , 2 1* SONG Lun & QIAO Xin 1
Tissue Engineering Research Center, Academy of Military Medical Sciences and Department of Neural Engineering and Biological
Interdisciplinary Studies, Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Beijing 100850, China; 2 Department of Neuroimmunology, Beijing Institute of Brain Sciences, Beijing 100850, China Received April 20, 2020; accepted May 11, 2020; published online September 7, 2020
The construction of engineered neural tissue using cell-derived extracellular matrix (ECM)-modified scaffolds is a promising approach. Silk fibroin (SF) sponge is an advantageous scaffold for the construction of engineered neural tissues, but it can still be modified to enhance its bioactivity. Inactivated mouse embryonic fibroblasts (MEFs) are proven that they can secrete amounts of ECM and soluble factors including neurotrophic factors. MEFs-derived ECM is expected to improve the activity of the scaffold. Here we aimed to construct 3D functional neural tissues based on MEFs-derived ECM modified SF sponge. MEFs were cultured on porous SF sponges and decellularized with TritonX-100 and NH4OH. The decellularized ECM deposited scaffolds were characterized through scanning electron microscopy and confocal microscopy. The efficiency of decellularization was evaluated by quantifying remaining DNA. Besides, we investigated the primary cortical neuronal growth and 3D neural network formation effect on MEFs-derived ECM modified SF sponges. Compared to bare SF sponges, ECM-SF sponges showed improved neuronal growth and axon extension indicated by immunofluorescence staining and RT-PCR. Specifically, ECM-SF sponges showed increased 3D neural network formation with functional connectivity. Hence, this study demonstrated that functionalization of SF scaffolds using cell-derived ECM could improve the bioactivity of SF materials and provide an ideal microenvironment for functional neural tissue formation. silk fibroin, cell-derived matrix, tissue engineering, neuron, scaffold Citation:
Zhu H M, Liu W, Yin Q, et al. Silk fibroin sponge combined with cell-derived ECM for tissue-engineered 3D functional neural tissues. Sci China Tech Sci, 2020, 63, https://doi.org/10.1007/s11431-020-1635-3
1 Introduction In recent years, neural tissue engineering has developed rapidly, and engineered neural tissues have been constructed by different strategies as a model for in vitro study or in vivo tissue regeneration. Several biofabrication strategies have been developed to remodel the structural and functional 3D neural tissue models [1]. Different 3D neural tissue models, such as scaffolds-based engineering models [2–9], and pluripotent stem cells-based organoids [10–12] have been *
Corresponding author (email: [email protected])
widely studied for their ability to generate neural tissues-like structures [13–
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