Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes
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ORIGINAL ARTICLE
Alterations in niban gene expression as a response to stress conditions in 3T3‑L1 adipocytes Mehtap Cevik1 · Meliha Koldemir Gunduz2 · Gokce Deliorman3 · Belgin Susleyici1 Received: 31 August 2020 / Accepted: 6 November 2020 © Springer Nature B.V. 2020
Abstract Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 µM linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600µM H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies. Keywords Niban · Linoleic acid · Cell stress · Obesity · Adipogenesis
Introduction Obesity is a chronic and inflammatory disease characterized by excessive fat accumulation in adipose tissue and leading to metabolism-related diseases such as lipid metabolism diseases, hypertension, atherosclerosis and ischemic heart diseases, as well as insulin resistance, type 2 diabetes, cardiovascular diseases and cancer [1, 2]. Although data from recent studies show that obesity is associated with stress signals, excessive lipid accumulation, disruptions in energy changes and inflammations in cells [3–5], the source of the stress has not been fully elucidated. * Belgin Susleyici [email protected] 1
Faculty of Arts and Sciences, Division of Biology, Department of Molecular Biology, Marmara University, Istanbul, Turkey
2
Department of Medical Services and Techniques, Kutahya Health Sciences University, Kutahya, Turkey
3
Faculty of Engineering and Architecture, Department of Software Engineering, Beykoz University, Istanbul, Turkey
Although the frequency of obesity is increasing worldwide, the available therapeutic options used in the treatment of obesity and obesity-related diseases remain limited and ineffective [1]. Therefore, understanding the molecular mechanisms related to the development of obesity is critical to obtain success in obesity treatment. In a recent study, presence of apoptosis has been reported in adipose tissue of obese humans and mice and the inh
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