An efficient protocol for in vitro propagation of Trichilia pallida Swartz (Meliaceae): a potential tree for natural ins
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RESEARCH NOTE
An efficient protocol for in vitro propagation of Trichilia pallida Swartz (Meliaceae): a potential tree for natural insecticide production Pamela de Souza Prim1 · Leandro Francisco de Oliveira2 · Luciana Lopes Fortes Ribas1 Received: 17 July 2020 / Accepted: 18 October 2020 © Springer Nature B.V. 2020
Abstract In vitro and ex vitro germination was successfully achieved for seedling production from monoembryonic and polyembryonic seeds. The seedlings were used as a source of explants for establishing a micropropagation protocol of Trichilia pallida. Cotyledonary node and apical shoot sections were cultured in MS medium supplemented with 6-benzyladenine (BA; 0, 2.0, 4.0, or 8.0 µM) during three subcultures in order to induce new shoots. Polyamines (PAs) were tested to promote shoot elongation and rooting. MS/2 medium supplemented with indole-3-butyric acid (IBA; 0, 1.25, 2.5, 5.0, and 10.0 µM) was used for root induction. The results indicated better responses of shoot regeneration when adding 2.0 µM BA to the culture medium, which promoted shoot regeneration (98% of explants and 5 to 7 shoots per explant), irrespective of the type of explant tested, during three subcultures. PAs showed a positive effect to promote shoot elongation with the supplementation of 0.5 mM spermidine (70%) and rooting of shoots with 2.0 mM putrescine (82%). Rooting was also achieved on MS/2 medium supplemented with 5.0 µM IBA, where 84% of explants showed root development. The commercial substrates tested in combination with vermiculite (1:1 v/v) were efficient for acclimatization, allowing 87.76% and 75.52% of survival rate after 90 and 120 days, respectively, in a greenhouse. In conclusion, a feasible and complete in vitro propagation protocol from seedlings was developed for T. pallida. Key message A complete protocol for germination and micropropagation of Trichilia pallida was established, a species with great potential for conservation and commercial purposes. Keywords Catiguá · Germination · Micropropagation · Polyamines · Polyembryonic seeds · Recalcitrant seeds Abbreviations BA 6-Benzyladenine IBA Indole-3-butyric acid MS Murashige and Skoog (1962) medium
PAs Polyamines PGRs Plant growth regulators Put Putrescine Spd Spermidine Spm Spermine
Communicated by Klaus Eimert. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11240-020-01958-4) contains supplementary material, which is available to authorized users. * Luciana Lopes Fortes Ribas [email protected] 1
Department of Botany, Federal University of Paraná, Av. Cel. Francisco H. dos Santos, 81530‑000 Curitiba, PR, Brazil
Laboratory of Plant Cell Biology, Department of Botany, Institute of Biosciences, University of São Paulo, Rua do Matão, 277, sala 107, 05508‑090 São Paulo, SP, Brazil
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Introduction Trichilia pallida Sw. popularly known as bat berry or catiguá is a tree species native to Brazil but not endemic, distributed throughout the Amazon Rainforest, Cerrado and Atlantic Forest (Flora do
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