An insecticide resistance diagnostic kit for whitebacked planthopper Sogatella furcifera (Horvath)
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ORIGINAL PAPER
An insecticide resistance diagnostic kit for whitebacked planthopper Sogatella furcifera (Horvath) Kaikai Mao1 • Zhijie Ren1 • Wenhao Li1 • Chaoya Liu1 • Pengfei Xu1 • Shun He1 • Jianhong Li1 Hu Wan1
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Received: 29 October 2019 / Revised: 22 July 2020 / Accepted: 31 August 2020 Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Sogatella furcifera (Horvath) is a notorious pest in most rice-producing regions of Asia, and evidence of resistance of S. furcifera to various insecticides has been documented in many areas. Given that the risk of insecticide resistance has spread, prospective monitoring should be expedited. Diagnostic tools for the rapid and accurate assessment of insecticide resistance are urgently needed to implement pest control and effective insecticide resistance management strategies. In the present study, a diagnostic kit based on the glass vial bioassay for the rapid detection of resistance to imidacloprid, nitenpyram, clothianidin, dinotefuran, thiamethoxam, isoprocarb and chlorpyrifos in S. furcifera was developed. The detection results can be obtained in 1 h, and the determination of C 90% mortality indicates sensitivity to insecticides. The insecticide resistance levels diagnosed by the kit were consistent with the results of the rice seedling dip method in field populations of S. furcifera. Moreover, the accuracy of the diagnosis of nitenpyram and chlorpyrifos resistance was further verified by field trials. The insecticide resistance diagnostic kit for S. furcifera, as a user-friendly tool (portable, short detection time), can be easily popularized. Its excellent performance qualifies the kit as a reliable screening tool to determine effective insecticides and a convenient addition to integrated pest management programs. Keywords Sogatella furcifera Insecticide resistance Diagnostic kit Accuracy verification
Key messages • A diagnostic kit for the rapid detection of resistance to imidacloprid, nitenpyram, clothianidin, dinotefuran, thiamethoxam, isoprocarb and chlorpyrifos in S. furcifera was developed.
Communicated by S. Downes. Kaikai Mao and Zhijie Ren have contributed equally to this paper.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10340-020-01277-9) contains supplementary material, which is available to authorized users. & Hu Wan [email protected] 1
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China
• The diagnostic results of the kit were consistent with those of the rice seedling dip method in field populations of S. furcifera, and the accuracy of the kit was further verified by field trials. • The kit can quickly detect the sensitivity of S. furcifera and guide the scientific selection of insecticides.
Introduction To ensure food security and reduce the yield loss caused by insect pests,
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