Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variet
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Development of InDel markers of Bph3 and pyramiding of four brown planthopper resistance genes into an elite rice variety Long He & Ling Zou & Qiuli Huang & Xichen Sheng & Weiren Wu & Jie Hu
Received: 29 April 2020 / Accepted: 21 September 2020 # Springer Nature B.V. 2020
Abstract Brown planthopper (BPH) is one of the most destructive pests in rice. Breeding resistant cultivars is the most effective strategy to control BPH. Bph3 is a broad-spectrum and durable BPH resistance locus spanning ~ 50 kb on the short arm of rice chromosome 4. Using the genomic variation data of 4726 rice varieties from public database, we developed 8 InDel markers within the Bph3 locus. By analyzing 563 rice varieties, we demonstrated that these markers could effectively identify Bph3-carriers, with an accuracy rate near to 90%. Through marker-assisted backcross breeding (MABC), we introduced four BPH resistance genes (Bph3/14/18/32) into an elite rice variety, Guang 8B. The results showed that Bph3 had the biggest effect, followed by Bph14 and Bph32, and Bph18 had the smallest effect. In addition, the level of BPH resistance increased with the number of genes pyramided. However, in the case of only two genes being pyramided, the resistance increase was not significant in comparison Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01175-z) contains supplementary material, which is available to authorized users. L. He : L. Zou : Q. Huang : X. Sheng : W. Wu : J. Hu Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture & Forestry University, Fuzhou, China L. He : L. Zou : Q. Huang : X. Sheng : W. Wu (*) : J. Hu (*) Fujian Key Laboratory of Crop Breeding by Design, Fujian Agriculture & Forestry University, Fuzhou, China e-mail: [email protected] e-mail: [email protected]
with the larger effect of the two genes. This suggests that the resistance effects are not simply additive, but epistasis may be involved. The InDel markers and pyramiding lines developed in this study will facilitate Bph3-carrier identification and BPH resistance breeding in rice. Keywords Rice . Bph3 . InDel markers . Gene pyramiding
Introduction Brown planthopper (BPH) is a kind of monophagous pest, which has the characteristics of long-distance migration and thermophily. It has strong migration ability and rapid propagation speed, which will cause a significant loss of rice yield, even result in total crop failure. In addition to its own harm, BPH is also one of a vector of rice virus such as rice ragged stunt virus (Cha et al. 2008). At present, BPH is mainly controlled by spraying insecticides, but the method has obvious drawbacks, such as high cost of pesticides and labors, and environmental pollution. In addition, long-term use of pesticides can enhance the resistance of BPH populations against pesticides (Jena and Kim, 2010). Therefore, utilization of rice-own resistance is considered to be the most effective and eco-friendly approach in the
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