An organic solvent-tolerant lipase of Streptomyces pratensis MV1 with the potential application for enzymatic improvemen
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ORIGINAL ARTICLE
An organic solvent-tolerant lipase of Streptomyces pratensis MV1 with the potential application for enzymatic improvement of n6/n3 ratio in polyunsaturated fatty acids from fenugreek seed oil Mahbobeh Vahidi1,2 • Somaye Imanparast1 • Hoda Jahandar2 • Hamid Forootanfar3 Somayeh Mojtabavi1 • Mohammad Ali Faramarzi1
•
Revised: 18 August 2020 / Accepted: 9 September 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract Lipase-catalyzed esterification is an efficient technique in the production of polyunsaturated fatty acid (PUFA) concentrates which are applied for nutrition and health purposes. In this project, a solvent-tolerant lipase from Streptomyces pratensis MV1 was immobilized and purified by a hydrophobic support. The purified lipase revealed enhanced activity and stability towards chemicals, organic solvents, and a broad range of pH values. The production of lipase was enhanced to 7.0 U/mL after optimization by a central composite design. Acylglycerols (AGs) rich in a-linolenic acid (45%, w/w) were produced and a favorable n-6/n-3 free fatty acid (FFA) ratio of 1.1 was achieved in fenugreek seed oil using the immobilized lipase. The ability of S. pratensis lipase in ester synthesis and the improvement of n6/n3 FFA ratio make it a suitable candidate in food production industries. Keywords n-6/n-3 free fatty acid ratio Polyunsaturated fatty acids Fenugreek seed oil Solvent tolerant lipase Streptomyces pratensis Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13197-020-04784-w) contains supplementary material, which is available to authorized users. & Mohammad Ali Faramarzi [email protected] 1
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, 1417614411 Tehran, Iran
2
Pharmaceutical Sciences Research Center, School of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
Introduction Polyunsaturated fatty acids (PUFAs) particularly a-linolenic acid (ALA) are health-promoting fatty acids, because of their role in reducing blood LDL cholesterol, regulation of inflammation, brain functions, fluidity or integrity of membrane bilayer structures, vasodilation, preventing cardiac arrhythmias, and reducing thrombosis by conversion to eicosanoids (Nicholson et al. 2013). Recent studies have shown that PUFAs can strengthen the immune system and increase resistance and recovery from virus diseases such as the Middle East Respiratory Syndrome (MERS) and the Severe Acute Respiratory Syndrome (SARS-CoV-2) infections (Galanakis 2020). The human body does not contain enzymes to biosynthesize ALA, so it should be supplied in the diet. One of the current strategies to develop PUFA functional foods is mainly focused on the addition of one or a combination of PUFAs from natural sources to nutrients to enhance their tota
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