Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines
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O R I GI N A L P A P E R
S. K. Naess á J. M. Bradeen á S. M. Wielgus G. T. Haberlach á J. M. McGrath á J. P. Helgeson
Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines Received: 6 November 2000 / Accepted: 23 January 2001 / Published online: 12 April 2001 Ó Springer-Verlag 2001
Abstract Somatic hybrids have been obtained between potato and Solanum bulbocastanum PI 245310, a Mexican diploid (2n=2x=24) species. Through restriction fragment length polymorphism (RFLP) and randomly ampli®ed polymorphic DNA (RAPD) analyses it was found that the somatic hybrids contain each chromosome of the diploid parent and that the synteny of RFLP markers noted with tomato, potato and S. brevidens is largely maintained in S. bulbocastanum. RFLP analyses of BC1 progeny of two dierent hybrids indicated that a substantial number of markers were either lost or were heterozygous, in marked contrast with results previously noted with S. brevidens. A RAPD map for all 12 chromosomes of S. bulbocastanum was prepared and marker transmission was followed in three BC2 populations. Results with chromosomes 3, 8 and 10 from these populations are compared. Keywords Somatic hybrid á potato á Solanum bulbocastanum á RFLP á RAPD
Introduction Many wild Solanum species related to potato (S. tuberosum) have novel disease resistances which may provide durable protection against pests and minimize the need for pesticide applications. Many of these speCommunicated by R. Hagemann S. K. Naess á J. M. Bradeen á S. M. Wielgus á G. T. Haberlach J. M. McGrath1 á J. P. Helgeson (&) USDA/ARS Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA E-mail: [email protected] Fax: + 1-608-262-1541 Present address: USDA-ARS, Sugar Beet and Bean Research Unit, 494 Plant and Soil Science Building, Michigan State University, East Lansing, MI 48824-1325 USA 1
cies cannot be crossed with potato and alternative means to combine the DNA of the wild and domesticated species are required. Somatic hybridization has been useful in this regard. Somatic hybrids between S. brevidens and S. tuberosum have provided a new source of resistance to Potato Leaf Roll Virus and Erwinia soft rots (Helgeson et al. 1986; Austin et al. 1988). Resistance to Potato Virus Y (PVY) can be obtained from somatic hybrids of potato and S. etuberosum (Novy and Helgeson 1994). S. commersonii and potato have been hybridized to give a new resistance to bacterial wilt disease caused by Ralstonia solanacearum (Laferriere et al. 1999). For the past few years new genotypes of Phytophthora infestans, the pathogen that causes late blight in potato, have caused signi®cant damage to the potato industry (Fry and Goodwin 1997). Somatic hybrids of potato and S. bulbocastanum, a Mexican species that is highly resistant to late blight, are resistant to these new genotypes of the pathogen (Helgeson et al. 1998). Late blight resistance from S. bulbocastanum, if stably captured in potato breeding lines, could greatly r
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