Analytical Ultracentrifugation Instrumentation, Software, and Applic
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a so
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Analytical Ultracentrifugation Instrumentation, Software, and Applications
Analytical Ultracentrifugation
Susumu Uchiyama • Fumio Arisaka • Walter F. Stafford • Tom Laue Editors
Analytical Ultracentrifugation Instrumentation, Software, and Applications
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Editors Susumu Uchiyama Graduate School of Engineering Osaka University Suita Osaka, Japan
Fumio Arisaka Life Science Research Center Nihon University Fujisawa Kanagawa, Japan
Walter F. Stafford Department of Neurology Harvard Medical School Boston Massachusetts, USA
Tom Laue BITC University of New Hampshire Durham New Hampshire, USA
ISBN 978-4-431-55983-2 DOI 10.1007/978-4-431-55985-6
ISBN 978-4-431-55985-6 (eBook)
Library of Congress Control Number: 2016938115 © Springer Japan 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer Japan KK
Preface
Analytical ultracentrifugation (AUC) is one of most powerful and reliable techniques to study the biophysical behavior of solutes in solution. Since 1950, there have been more than 16,000 references to analytical ultracentrifugation, including 17 books on the topic. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. In this book, therefore, we focus on (1) providing chapters that cover everything essential for beginners to the most advanced users; (2) updating the field on advances in hardware, software, and applications; and (3) encompassing AUC applications for nonbiological questions. The first two chapters (Chaps. 1 and 2) in Part 1 provide minimum but essential theoretical and experimental explanations for studies using AUC. In Part 2, novel analytical ultracentrifuge instrumentation (Chap. 3) and detection systems (Chaps. 4 and 5) that give added dimensions to AUC are introduced, followed by three chapters describing computer programs for sedimentation data analysis (Chaps. 6, 7 and8). Th
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