Application of Atomic Dielectric Resonance Spectroscopy for the screening of blood samples from patients with clinical v
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BioMed Central
Open Access
Research
Application of Atomic Dielectric Resonance Spectroscopy for the screening of blood samples from patients with clinical variant and sporadic CJD Timothy J Fagge1, G Robin Barclay*2, G Colin Stove3, Gordon Stove3, Michael J Robinson3, Mark W Head1, James W Ironside1 and Marc L Turner2 Address: 1National CJD Surveillance Unit & Division of Pathology, University of Edinburgh School of Molecular and Clinical Medicine, Western General Hospital, Edinburgh EH4 2XU, UK, 2SNBTS Adult Cell Therapy Group, Scottish Centre for Regenerative Medicine, University of Edinburgh School of Clinical Sciences, The Chancellor's Building, 49 Little France Crescent, Edinburgh EH16 4SB, UK and 3ADROK Ltd (formerly Radar World Ltd), Waterloo House, 17 Waterloo Place, Edinburgh, EH1 3BG, UK Email: Timothy J Fagge - [email protected]; G Robin Barclay* - [email protected]; G Colin Stove - [email protected]; Gordon Stove - [email protected]; Michael J Robinson - [email protected]; Mark W Head - [email protected]; James W Ironside - [email protected]; Marc L Turner - [email protected] * Corresponding author
Published: 30 August 2007 Journal of Translational Medicine 2007, 5:41
doi:10.1186/1479-5876-5-41
Received: 13 July 2007 Accepted: 30 August 2007
This article is available from: http://www.translational-medicine.com/content/5/1/41 © 2007 Fagge et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Sub-clinical variant Creutzfeldt-Jakob disease (vCJD) infection and reports of vCJD transmission through blood transfusion emphasise the need for blood screening assays to ensure the safety of blood and transplanted tissues. Most assays aim to detect abnormal prion protein (PrPSc), although achieving required sensitivity is a challenge. Methods: We have used innovative Atomic Dielectric Resonance Spectroscopy (ADRS), which determines dielectric properties of materials which are established by reflectivity and penetration of radio/micro waves, to analyse blood samples from patients and controls to identify characteristic ADR signatures unique to blood from vCJD and to sCJD patients. Initial sets of blood samples from vCJD, sCJD, non-CJD neurological diseases and normal healthy adults (blood donors) were screened as training samples to determine group-specific ADR characteristics, and provided a basis for classification of blinded sets of samples. Results: Blood sample groups from vCJD, sCJD, non-CJD neurological diseases and normal healthy adults (blood donors) screened by ADRS were classified with 100% specificity and sensitivity, discriminating these by a co-variance expert analysis system. Conclusion: ADRS appears capable of recognising and discriminating serum samples from
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